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Rockland
ERK2 Polyclonal Antibody
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FIGURE: 1 / 1
ERK2 Antibody (100-401-218) in WB
Western blot using Rocklands Affinity Purified anti-p42 MAP Kinase (ERK2) antibody shows detection of ERK2 in several whole cell lysates: HeLa (lane 1), A431 (lane 2), and NIH3T3 (lane 3). Detection occurs using a 1:1,000 dilution of the primary antibody followed by 1:4,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure was approximately 1. Other detection systems will yield similar results.
Product Details
100-401-218
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Erk2 antibody was prepared by repeated immunizations with an Erk2 containing fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
Conjugate
Form
Liquid
Concentration
85 mg/mL
Amount
8.5 mg
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antiserum is directed against human p42 MAP Kinase (ERK2) protein and is useful in determining its presence by immunoblotting. No reactivity is observed against p44 MAP Kinase (ERK1). Cross reactivity is expected with p44 MAP Kinase (ERK1) proteins from human and mouse sources. Reactivity to rat tissues is also anticipated due to high sequence homology. Reactivity against homologues from other sources is not known.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ERK-2; Erk2; cancer susceptibility protein; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 2; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p42-MAPK; protein tyrosine kinase ERK2; unnamed protein product
Gene Aliases: ERK; ERK-2; ERK2; ERT1; MAPK1; MAPK2; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2
UniProt ID: (Human) P28482
Entrez Gene ID: (Human) 5594
nucleotide binding
phosphotyrosine binding
DNA binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
transferase activity
phosphatase binding
identical protein binding
protein serine kinase activity
MAPK cascade
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
apoptotic process
chemotaxis
cellular response to DNA damage stimulus
signal transduction
cell surface receptor signaling pathway
epidermal growth factor receptor signaling pathway
chemical synaptic transmission
heart development
learning or memory
insulin receptor signaling pathway
organ morphogenesis
positive regulation of macrophage chemotaxis
positive regulation of peptidyl-threonine phosphorylation
neural crest cell development
Schwann cell development
peptidyl-threonine phosphorylation
cytosine metabolic process
regulation of ossification
regulation of cellular pH
thyroid gland development
regulation of protein stability
lipopolysaccharide-mediated signaling pathway
positive regulation of telomere maintenance
response to lipopolysaccharide
regulation of stress-activated MAPK cascade
mammary gland epithelial cell proliferation
cellular response to amino acid starvation
response to nicotine
intracellular signal transduction
ERBB signaling pathway
ERBB2-ERBB3 signaling pathway
outer ear morphogenesis
myelination
response to exogenous dsRNA
positive regulation of cholesterol biosynthetic process
negative regulation of cell differentiation
insulin-like growth factor receptor signaling pathway
thymus development
T cell receptor signaling pathway
B cell receptor signaling pathway
stress-activated MAPK cascade
regulation of cytoskeleton organization
Bergmann glial cell differentiation
long-term synaptic potentiation
face development
lung morphogenesis
trachea formation
labyrinthine layer blood vessel development
cardiac neural crest cell development involved in heart development
interleukin-34-mediated signaling pathway
chemokine-mediated signaling pathway
ERK1 and ERK2 cascade
response to epidermal growth factor
cellular response to tumor necrosis factor
caveolin-mediated endocytosis
regulation of Golgi inheritance
positive regulation of macrophage proliferation
positive regulation of neuroinflammatory response
regulation of early endosome to late endosome transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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