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ERN1 Polyclonal Antibody

View all (52) IRE1 alpha antibodies

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ERN1 Polyclonal Antibody

Product Details

ER1902-90

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within N-terminal Human ERN1.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.4, with 0.2% BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; Ire1p; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1; unnamed protein product

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Gene Aliases: ERN1; hIRE1p; IRE1; IRE1a; IRE1P; RGD1559716

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UniProt ID: (Human) O75460

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Entrez Gene ID: (Human) 2081, (Rat) 498013

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Function(s)
nucleotide binding magnesium ion binding catalytic activity endonuclease activity endoribonuclease activity ribonuclease activity protein kinase activity protein serine/threonine kinase activity platelet-derived growth factor receptor binding protein binding ATP binding kinase activity transferase activity hydrolase activity enzyme binding Hsp70 protein binding identical protein binding protein homodimerization activity ADP binding metal ion binding unfolded protein binding Hsp90 protein binding protein serine kinase activity endoribonuclease
Process(es)
endothelial cell proliferation mRNA processing RNA catabolic process mRNA catabolic process protein phosphorylation apoptotic process response to unfolded protein negative regulation of gene expression regulation of macroautophagy endoplasmic reticulum unfolded protein response positive regulation of RNA splicing cellular response to unfolded protein response to endoplasmic reticulum stress cellular response to vascular endothelial growth factor stimulus IRE1-mediated unfolded protein response positive regulation of JNK cascade mRNA splicing, via endonucleolytic cleavage and ligation intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to glucose stimulus positive regulation of endoplasmic reticulum unfolded protein response insulin metabolic process positive regulation of vascular smooth muscle cell proliferation positive regulation of response to endoplasmic reticulum stress mRNA cleavage activation of signaling protein activity involved in unfolded protein response cell cycle arrest RNA splicing mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response protein autophosphorylation mRNA endonucleolytic cleavage involved in unfolded protein response RNA phosphodiester bond hydrolysis, endonucleolytic peptidyl-serine trans-autophosphorylation
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