Invitrogen

ERP29 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

ERP29 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ERp29 using anti-ERp29 polyclonal antibody (Product # PA3-011) shows staining in HMVEC Cells.

ERP29 Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MDCK (Lane 1), NIH/3T3 (Lane 2), Hep G2 (Lane 3), A-549 (Lane 4), MCF-7 (Lane 5), A-431 (Lane 6) and HEK-293 (Lane 7). The blot was probed with Anti-ERp29 Rabbit polyclonal Antibody (Product # PA3-011, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 29 kDa band corresponding to ERp29 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

PA3-011

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1:500	View 1 publication 1 publication
Immunoprecipitation (IP)	1:500	-

Product Specifications
Species Reactivity	Hamster, Human, Mouse, Non-human primate, Rat, Bovine, Dog, Guinea pig
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	6-histidine epitope tagged full length recombinant rat ERp29. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2101510

Product Specific Information

PA3-011 detects ERp29 protein in rat, mouse, hamster, canine, human, non-human primate, bovine and guinea pig samples.

PA3-011 has successfully been used in immunofluorescence, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~29 kDa protein representing ERp29 in rat liver homogenate. Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using PA3-011.

The PA3-011 immunogen was a 6-histidine epitope tagged full length recombinant ERp29 protein.

Target Information

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Peripheral nerve axons contain machinery for co-translational secretion of axonally-generated proteins.

Neuroscience bulletin

Merianda T,Twiss J

PA3-011 was used in immunocytochemistry to study the co-translational secretion of proteins synthesized in peripheral nerve axons

Thu Aug 01 00:00:00 EDT 2013

PKR-like endoplasmic reticulum kinase (PERK) activation following brain ischemia is independent of unfolded nascent proteins.

Neuroscience

Sanderson TH,Deogracias MP,Nangia KK,Wang J,Krause GS,Kumar R

PA3-011 was used in western blot to investigate the independence of PERK activation from unfolded nascent proteins

Wed Sep 01 00:00:00 EDT 2010

Differential cell death regulation between adult-unloaded and aged rat soleus muscle.

Mechanisms of ageing and development

Ogata T,Machida S,Oishi Y,Higuchi M,Muraoka I

PA3-011 was used in western blot to study the different regulation of cell death in aged atrophy and un-load atrophy in rat skeletal muscle

Fri May 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: endoplasmic reticulum lumenal protein ERp28; endoplasmic reticulum protein 29; Endoplasmic reticulum protein ERp29; Endoplasmic reticulum resident protein 28; Endoplasmic reticulum resident protein 29; Endoplasmic reticulum resident protein 31; endoplasmic retuclum protein 29; epididymis secretory protein Li 107; ERp28; ERp29; ERp31; protein disulfide isomerase family A, member 9

Gene Aliases: 1200015M03Rik; 2810446M09Rik; AW209030; C12orf8; ERP28; ERP29; ERp31; HEL-S-107; PDI-DB; PDIA9

UniProt ID: (Bovine) P81623, (Human) P30040, (Rat) P52555, (Mouse) P57759

Entrez Gene ID: (Bovine) 613357, (Dog) 477482, (Human) 10961, (Chinese hamster) 100762501, (Guinea pig) 100731699, (Rat) 117030, (Mouse) 67397

Molecular Function: membrane traffic protein