|Western Blot (WB)||1:100-1:1000|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.01% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-007 detects ERp72 protein in human and mouse samples.
PA1-007 has successfully been used in ICC, IF and Western blot procedures. By Western blot, this antibody detects a 72 kDa protein representing ERp72 in human fibrosarcoma HT 1080 cell lysate.
The PA1-007 immunogen is a synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72. This peptide (Cat. # PEP-223) is available for use in neutralization and control experiments.
ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: calcium binding protein, intestinal; EC 18.104.22.168; endoplasmic reticulum protein 72; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; protein disulfide isomerase family A, member 4; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related); protein disulfide isomerase-associated 4; Protein disulfide-isomerase A4; Protein ERp-72; testicular tissue protein Li 137
Gene Aliases: AI987846; Cai; ERp-72; ERP70; ERP72; PDIA4
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