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Immunofluorescent analysis of ERp72 (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a ERp72 rabbit polyclonal antibody (Product # PA1-007), at a concentration of 10ug/ml in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-007 detects ERp72 protein in human and mouse samples.
PA1-007 has successfully been used in ICC, IF and Western blot procedures. By Western blot, this antibody detects a 72 kDa protein representing ERp72 in human fibrosarcoma HT 1080 cell lysate.
The PA1-007 immunogen is a synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72. This peptide (Cat. # PEP-223) is available for use in neutralization and control experiments.
ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
calcium binding protein, intestinal; EC 220.127.116.11; endoplasmic reticulum protein 72; endoplasmic reticulum resident protein 70; endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; protein disulfide isomerase family A, member 4; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related); protein disulfide isomerase-associated 4; protein disulfide-isomerase A4; Protein ERp-72; testicular tissue protein Li 137
AI987846; Cai; ERp-72; ERP70; ERP72; PDIA4