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ETFA Antibody (PA5-28201) in IHC (P)
ETFA Polyclonal Antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ETFA Polyclonal Antibody (Product # PA5-28201) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-28201
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 272 of Human ETFA
Conjugate
Form
Liquid
Concentration
0.88 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545677
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, PC-12.^M
^M
Predicted reactivity: Mouse (93%), Rat (92%), Cat (94%), Pig (94%), Chicken (81%), Rhesus Monkey (97%), Bovine (94%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-ETF; electron transfer flavoprotein alpha-subunit; Electron transfer flavoprotein subunit alpha, mitochondrial; electron transfer flavoprotein, alpha polypeptide; electron transferring flavoprotein, alpha polypeptide; electron-transfer-flavoprotein alpha polypeptide; glutaric aciduria II
Gene Aliases: 2010200I21Rik; D9Ertd394e; EMA; ETF; ETFA; GA2; MADD
UniProt ID: (Human) P13804, (Mouse) Q99LC5, (Rat) P13803
Entrez Gene ID: (Human) 2108, (Mouse) 110842, (Rat) 300726
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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