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Invitrogen
ETS2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
ETS2 Antibody (PA5-28053) in ICC/IF
Immunofluorescence analysis of ETS2 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ETS2 Polyclonal Antibody (Product # PA5-28053) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000 dilution) for 45 mi... View More
Product Details
PA5-28053
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 287 of Human ETS2
Conjugate
Form
Liquid
Concentration
0.35 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545529
Product Specific Information
Recommended positive controls: HeLa.^M
^M
Predicted reactivity: Mouse (87%), Rat (88%), Bovine (89%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: avian erythroblastosis virus E26 (v-ets) oncogene homolog 2; E26 avian leukemia oncogene 2, 3' domain; oncogene ETS-2; Protein C-ets-2; v-ets avian erythroblastosis virus E2 oncogene homolog 2; v-ets avian erythroblastosis virus E26 oncogene homolog 2; v-ets erythroblastosis virus E26 oncogene homolog 2
Gene Aliases: ETS2; ETS2IT1
UniProt ID: (Human) A6NM68
Entrez Gene ID: (Human) 2114, (Rat) 304063
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
glucocorticoid receptor binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
ectodermal cell fate commitment
transcription, DNA-templated
mesoderm development
cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
primitive streak formation
transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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