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Invitrogen
EVI1 Polyclonal Antibody
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FIGURE: 1 / 2
EVI1 Antibody (PA5-96384) in WB
Western blot analysis of EVI1 in various lysates. Samples were incubated with EVI1 Polyclonal antibody (Product # PA5-96384) using a dilution of 1:2,500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-96384
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MECOM (NP_0049822)
Conjugate
Form
Liquid
Concentration
0.34 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse kidney, Mouse heart; Cellular Location: Nucleus, Nucleus speckle
Immunogen sequence: MRSKGRARKL ATNNECVYGN YPEIPLEEMP DADGVASTPS LNIQEPCSPA TSSEAFTPKE GSPYKAPIYI PDDIPIPAEF ELRESNMPGA GLGIWTKRKI EVGEKFGPYV GEQRSNLKDP SYGWEILDEF YNVKFCIDAS QPDVGSWLKY IRFAGCYDQH NLVACQINDQ IFYRVVADIA PGEELLLFMK
Target Information
EVI1 (MECOM) is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, cell differentiation, and proliferation. It can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. The EVI1 gene can undergo translocation with the AML1 gene, resulting in overexpression of the EVI1 gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AML1-EVI-1 fusion protein; ecotropic viral integration site 1; Ecotropic virus integration site 1 protein homolog; EVI-1; Histone-lysine N-methyltransferase MECOM; MDS1 and EVI1 complex locus protein; MDS1 and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex locus protein MDS1; MGC163392; MGC97004; myelodysplasia syndrome 1 homolog; Myelodysplasia syndrome 1 protein; Myelodysplasia syndrome-associated protein 1; oncogene EVI1; OTTHUMP00000213163; OTTHUMP00000213164; OTTHUMP00000213165; OTTHUMP00000213187; OTTHUMP00000213192; PR domain 3; zinc finger protein Evi1
Gene Aliases: AML1-EVI-1; EVI1; MDS1; MDS1-EVI1; MECOM; PRDM3; RUSAT2
UniProt ID: (Human) Q03112
Entrez Gene ID: (Human) 2122, (Rat) 294924
transcription, DNA-templated
apoptotic process
cell differentiation
histone lysine methylation
negative regulation of programmed cell death
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of JNK cascade
regulation of cell cycle
hematopoietic stem cell proliferation
in utero embryonic development
neutrophil homeostasis
inflammatory response
response to bacterium
post-embryonic development
stem cell population maintenance
negative regulation of transforming growth factor beta receptor signaling pathway
forebrain development
embryonic forelimb morphogenesis
embryonic hindlimb morphogenesis
regulation of cell proliferation
positive regulation of transcription from RNA polymerase II promoter
pericardium development
renal system development
positive regulation of brown fat cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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