Invitrogen

EXO1 Polyclonal Antibody

View all (14) EXO1 antibodies

Questions & Answers

Questions & Answers

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EXO1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of EXO1 was performed in HCT116 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: EXO1 Polyclonal Antibody (Product # PA5-29058) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

EXO1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Exonuclease 1 in paraformaldehyde-fixed HeLa cells using an Exonuclease 1 polyclonal antibody (Product # PA5-29058) at a 1:500 dilution.

EXO1 Antibody in Western Blot (WB) — Western Blot analysis of EXO1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EXO1 Polyclonal Antibody (Product # PA5-29058) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

EXO1 Antibody in Western Blot (WB) — Western blot analysis of Exonuclease 1 using 50 µg of mouse thymus lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Exonuclease 1 polyclonal antibody (Product # PA5-29058) at a dilution of 1:1000.

Product Details

PA5-29058

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Mouse, Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 474 and 753 of Human Exonuclease 1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.72 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% Proclin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2546534

Product Specific Information

Recommended positive controls: Jurkat, NCI-H929, Mouse thymus.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Required for muscle atrophy following denervation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Exonuclease 1; Exonuclease I; hExo1; hExoI; mExo1; rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1

Gene Aliases: 5730442G03Rik; EXO1; EXOI; HEX1; hExoI; Msa

UniProt ID: (Human) Q9UQ84, (Mouse) Q9QZ11

Entrez Gene ID: (Human) 9156, (Mouse) 26909

Molecular Function: exodeoxyribonuclease

DNA synthesis involved in DNA repair strand displacement humoral immune response mediated by circulating immunoglobulin DNA replication DNA repair mismatch repair DNA recombination somatic hypermutation of immunoglobulin genes isotype switching meiotic cell cycle RNA phosphodiester bond hydrolysis, endonucleolytic regulation of signal transduction by p53 class mediator immune system process nucleobase-containing compound metabolic process cellular response to DNA damage stimulus nucleic acid phosphodiester bond hydrolysis

Process(es)

DNA binding chromatin binding RNA-DNA hybrid ribonuclease activity exonuclease activity protein binding 5'-3' exonuclease activity 5'-3' exodeoxyribonuclease activity single-stranded DNA 5'-3' exodeoxyribonuclease activity metal ion binding flap endonuclease activity double-stranded DNA 5'-3' exodeoxyribonuclease activity catalytic activity nuclease activity endonuclease activity hydrolase activity hydrolase activity, acting on ester bonds phosphoric ester hydrolase activity structure-specific DNA binding T/G mismatch-specific endonuclease activity retroviral 3' processing activity