Invitrogen

EXO1 Polyclonal Antibody

View all (14) EXO1 antibodies

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Questions & Answers

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EXO1 Antibody in Western Blot (WB) — Western blot was performed using Anti-EXO1 Polyclonal Antibody (Product # PA5-86470) and a 115 kDa band corresponding to Exonuclease 1 was observed across cell lines. Whole cell extracts (60 µg lysate) of IMR-32 (Lane 1), PANC-1 (Lane 2), Jurkat (Lane 3), HeLa (Lane 4) in Fig a and HEK-293 (Lane 1), HEK-293 treated with Camptothecin (1µM) for 2 hrs (Lane 2) in Fig b, were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Upon treatment with Camptothecin the expression of EXO1 should decrease, which was observed in fig b, Lane 2.

EXO1 Antibody in Western Blot (WB) — Western blot analysis of EXO1 in Lane 1: rat testis tissue lysate (40 µg), Lane 2: Testis tissue lysate of mouse (40 µg), Lane 3: K562 whole cell lysate (10 µg), Lane 4: HEK293T whole cell lysate (10 µg). Sample was incubated with EXO1 polyclonal antibody (Product # PA5-86470) using a 1:500 dilution.

Product Details

PA5-86470

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide, corresponding to amino acids 55-05 of Human Exo1. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2803248

Target Information

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Required for muscle atrophy following denervation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Exonuclease 1; Exonuclease I; hExo1; hExoI; mExo1; rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1

Gene Aliases: 5730442G03Rik; EXO1; EXOI; HEX1; hExoI; Msa

UniProt ID: (Human) Q9UQ84, (Mouse) Q9QZ11

Entrez Gene ID: (Human) 9156, (Rat) 305000, (Mouse) 26909

Molecular Function: exodeoxyribonuclease

DNA synthesis involved in DNA repair strand displacement humoral immune response mediated by circulating immunoglobulin DNA replication DNA repair mismatch repair DNA recombination somatic hypermutation of immunoglobulin genes isotype switching meiotic cell cycle RNA phosphodiester bond hydrolysis, endonucleolytic regulation of signal transduction by p53 class mediator DNA catabolic process, endonucleolytic DNA catabolic process, exonucleolytic biological_process immune system process nucleobase-containing compound metabolic process cellular response to DNA damage stimulus nucleic acid phosphodiester bond hydrolysis

Process(es)

DNA binding chromatin binding RNA-DNA hybrid ribonuclease activity exonuclease activity protein binding 5'-3' exonuclease activity 5'-3' exodeoxyribonuclease activity single-stranded DNA 5'-3' exodeoxyribonuclease activity metal ion binding flap endonuclease activity double-stranded DNA 5'-3' exodeoxyribonuclease activity molecular_function structure-specific DNA binding catalytic activity nuclease activity endonuclease activity hydrolase activity hydrolase activity, acting on ester bonds phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity