|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||N-terminus (aa1-343) of the mouse Ezh2 protein|
|Storage buffer||whole serum|
|Contains||0.0015-0.05% Proclin 300, <0.1% thimerosal|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Histone-modifying genes as biomarkers in hepatocellular carcinoma.
49-1043 was used in immunohistochemistry - paraffin section to correlate histone-modifying genes and prognosis of hepatocellular carcinoma to identify out new diagnostic markers
|Hung SY,Lin HH,Yeh KT,Chang JG||International journal of clinical and experimental pathology (7:2496)||2014|
The central role of EED in the orchestration of polycomb group complexes.
49-1043 was used in ChIP assay and western blot to study the Involvement of EED in the organization of polycomb group complexes
|Cao Q,Wang X,Zhao M,Yang R,Malik R,Qiao Y,Poliakov A,Yocum AK,Li Y,Chen W,Cao X,Jiang X,Dahiya A,Harris C,Feng FY,Kalantry S,Qin ZS,Dhanasekaran SM,Chinnaiyan AM||Nature communications (5:null)||2014|
CCN3/NOV gene expression in human prostate cancer is directly suppressed by the androgen receptor.
49-1043 was used in ChIP assay to report a tumor-suppressive role of NOV in prostate cancer.
|Wu L,Runkle C,Jin HJ,Yu J,Li J,Yang X,Kuzel T,Lee C,Yu J||Oncogene (33:504)||2014|
|Human||1:1000||Enhancer of Zeste homolog 2 (EZH2) is overexpressed in recurrent nasopharyngeal carcinoma and is regulated by miR-26a, miR-101, and miR-98.||Alajez NM,Shi W,Hui AB,Bruce J,Lenarduzzi M,Ito E,Yue S,O'Sullivan B,Liu FF||Cell death and disease (1:null)||2011|
|Human||1:100||EZH2 regulates expression of p57 and contributes to progression of ovarian cancer in vitro and in vivo.||Guo J,Cai J,Yu L,Tang H,Chen C,Wang Z||Cancer science (102:530)||2011|