|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of human EZH2 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate carcinoma.
KMT6 / Enhancer of zeste homologue 2 (EZH2) is a histone lysine methyl transferase which is associated with transcriptional repression. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. The expression of EZH2 was also seen in follicular T-cells at different stages during T-cell differentiation and is highly expressed in a variety of tumors including lymphoma, breast and prostate. EZH2 is involved in the progression of prostate canc
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.