Proteintech

Endoglin/CD105 Polyclonal Antibody

View all (103) CD105 (Endoglin) antibodies

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Endoglin/CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Endoglin/CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Endoglin/CD105 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Endoglin/CD105 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Endoglin/CD105 Antibody in Immunoprecipitation (IP) — IP result of anti-Endoglin/CD105 (IP:10862-1-AP, 3ug; Detection:10862-1-AP 1:300) with mouse lung tissue lysate 4000ug.

Product Details

10862-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:2,000-1:8,000	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Bovine, Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Endoglin/CD105 Fusion Protein Ag1287 (331-658 aa encoded by BC014271) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: GGRLQTSPA PIQTTPPKDT CSPELLMSLI QTKCADDAMT LVLKKELVAH LKCTITGLTF WDPSCEAEDR GDKFVLRSAY SSCGMQVSAS MISNEAVVNI LSSSSPQRKK VHCLNMDSLS FQLGLYLSPH FLQASNTIEP GQQSFVQVRV SPSVSEFLLQ LDSCHLDLGP EGGTVELIQG RAAKGNCVSL LSPSPEGDPR FSFLLHFYTV PIPKTGTLSC TVALRPKTGS QDQEVHRTVF MRLNIISPDL SGCTSKGLVL PAVLGITFGA FLIGALLTAA LWYIYSHTRS PSKREPVVAV AAPASSESSS TNHSIGSTQS TPCSTSSMA (331-658 aa encoded by BC014271)

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.

Journal of experimental zoology. Part B, Molecular and developmental evolution

Zhao XX,An XL,Zhu XC,Jiang Y,Zhai YH,Zhang S,Cai NN,Tang B,Li ZY,Zhang XM

Sat Dec 01 00:00:00 EST 2018

MMP-14 aggravates onset of severe preeclampsia by mediating soluble endoglin release.

European review for medical and pharmacological sciences

Zhang XH,Zhang HY,Lu S,Jiang LL,Wu J,Yang YL,Zhang SA

Thu Mar 01 00:00:00 EST 2018

MMP-14 aggravates onset of severe preeclampsia by mediating soluble endoglin release.

European review for medical and pharmacological sciences

Zhang XH,Zhang HY,Lu S,Jiang LL,Wu J,Yang YL,Zhang SA

Thu Mar 01 00:00:00 EST 2018

Imbalance of angiogenic factors and avascular edematous cystic villi in a trisomy 13 pregnancy: a case report.

Placenta

Kakigano A,Mimura K,Kanagawa T,Nakayama M,Kanayama T,Fujita S,Kinugasa-Taniguchi Y,Endo M,Tomimatsu T,Kimura T

Mon Jul 01 00:00:00 EDT 2013

Placental expression of anti-angiogenic proteins in mirror syndrome: a case report.

Placenta

Graham N,Garrod A,Bullen P,Heazell AE

Fri Jun 01 00:00:00 EDT 2012

Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells.

Molecular human reproduction

Tskitishvili E,Sharentuya N,Temma-Asano K,Mimura K,Kinugasa-Taniguchi Y,Kanagawa T,Fukuda H,Kimura T,Tomimatsu T,Shimoya K

Mon Mar 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD105; CD105 antigen; Cell surface MJ7/18 antigen; Endoglin; ORW; transmembrane glycoprotein

Gene Aliases: AI528660; AI662476; CD105; Edg; END; Endo; ENG; HHT1; ORW1; S-endoglin

UniProt ID: (Human) P17813, (Mouse) Q63961

Entrez Gene ID: (Human) 2022, (Mouse) 13805, (Rat) 497010

Molecular Function: transmembrane signal receptor

chronological cell aging patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation heart looping sprouting angiogenesis endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated cell adhesion negative regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis negative regulation of cell migration BMP signaling pathway positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation dorsal aorta morphogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis endocardial cushion to mesenchymal transition positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation negative regulation of transcription from RNA polymerase II promoter angiogenesis response to hypoxia negative regulation of endothelial cell proliferation positive regulation of systemic arterial blood pressure protein phosphorylation transmembrane receptor protein serine/threonine kinase signaling pathway transforming growth factor beta receptor signaling pathway heart development positive regulation of gene expression cell migration extracellular matrix disassembly positive regulation of collagen biosynthetic process intracellular signal transduction wound healing cell motility negative regulation of nitric-oxide synthase activity cell chemotaxis negative regulation of pathway-restricted SMAD protein phosphorylation extracellular matrix constituent secretion detection of hypoxia response to corticosteroid response to statin bone development cellular response to mechanical stimulus response to transforming growth factor beta

Process(es)

transmembrane signaling receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity activin binding transforming growth factor beta binding transforming growth factor beta-activated receptor activity