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Immunofluorescent analysis of Endostatin (green) showing positive staining in the secretion of 293T cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endostatin polyclonal antibody (Product # PA1-601) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
|Tested species reactivity||Human , Mouse|
|Published species reactivity||Primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: R(129) R L M/T E S Y C E T W R T E(142)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-601 detects recombinant human and mouse endostatin.
PA1-601 has been successfully used in Western blot, IF/ICC and IHC (P) procedures. By Western blot, this antibody detects a 20 kDa protein representing recombinant human endostatin.
PA1-601 immunizing peptides are two peptides derived from mouse and human endostatin protein corresponding to amino acid residues 129-142 of endostatin.
Endostatin is a 20-kDa proteolytic COOH-terminal fragment derived from collagen XVIII. Endostatin reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene transfer of endostatin enhances the efficacy of doxorubicin to suppress human hepatocellular carcinomas in mice.
PA1-601 was used in western blot to investigate the effect of gene transfer of endostatin on the therapeutic efficacy of doxorubicin against human hepatocellular carcinomas
|Liu F,Tan G,Li J,Dong X,Krissansen GW,Sun X||Cancer science (98:1381)||2007|
KS, KNO, KNO1
antiangiogenic agent, collagen alpha-1(XVIII) chain, endostatin, multi-functional protein MFP, procollagen, type XVIII, alpha 1, Alpha 1 Collagen type 18 (XVIII)(COL18A1), COL18A1, Endostatin, KNO