Immunohistochemistry analysis of Endothelin-2 was performed on human lung tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a Endothelin-2 polyclonal antibody (Product # PA3-002) at a dilution of 1:3000, overnight at 4C in a humidified chamber. Tissues were washed, and detection was performed using an ABC kit composed of biotinylated goat anti-rabbit IgG, peroxidase-conjugated avidin, and 3-amino-9-ethylcarbazole (AEC) substrate in acetate buffer. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated Cys-S-Abu-SSWLDKE-Abu-VYF-Abu-HLDIIW|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IHC (P) analysis shows positive staining of endothelin-2 in human lung tissue.
Endothelins are endothelium-derived vasoconstrictor peptides. Endothelin 2, also known as ET-2, is a protein that in humans is encoded by the EDN2 gene. The secreted peptide (~2kD, 21 amino acids) is produced as a result of endothelin-2 precursor (~20 kD) processing and functions as a ligand for endothelin receptors which upon peptide binding modulates a wide array of cell signaling events. Diseases associated with endothelins include aortic disease and patent ductus arteriosus.
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