Invitrogen

Endothelin A Receptor Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 Published Figures

7 References

View all (7) Endothelin A Receptor antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Endothelin A Receptor Antibody in Western Blot (WB)

FIGURE: 1 / 9

Endothelin A Receptor Antibody (PA3-065) in WB

Western blot was performed using Anti-Endothelin A Receptor Polyclonal Antibody (Product # PA3-065) and a 50kDa band corresponding to Endothelin A Receptor was observed across tissues tested. Tissue extracts (40 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Lung (Lane 5), Rat Lung (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Pro... View More

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Endothelin A Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry was performed on acetone-fixed frozen mouse skin tissue sections. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (panel A), and Endothelin Receptor A polyclonal antibody (Product # PA3-065, panel B), or an Endothelin Receptor B polyclonal antibody (Product # PA3-066, panel C) all at dilutions of 1:800 overnight at 4C. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy. Data courtesy of the Innovators Program.

Endothelin A Receptor Antibody in Immunohistochemistry (IHC) — Immunohistochemistry of ETA receptors in the atrium of mouse heart.

Endothelin A Receptor Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Endothelin A Receptor in ETA-transfected cells using a polyclonal antibody (Product # PA3-065).

Endothelin A Receptor Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Mouse Heart, Rat Heart, Mouse Cerebellum, Rat Cerebellum, Mouse Lung, Rat Lung owing to their inherent genetic constitution. Relative expression of Endothelin A Receptor was observed in Mouse and Rat Heart as compared to Cerebellum, Lung using Anti-Endothelin A Receptor Polyclonal Antibody (Product # PA3-065) in Western Blot. {RE}

Product Details

PA3-065

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:500-1:2,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:5,000

View 1 publication 1 publication

Immunoelectrophoresis (IE)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

COOH-terminal amino acids of mouse ETA (408-427) KNQEQNNHNTERSSHKDSMN coupled to KLH

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_11153077

Product Specific Information

PA3-065 detects Endothelin Receptor A from human, mouse, and rat samples.

PA3-065 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures. By western blot, this antibody detects a 46kDa protein corresponding to mouse Endothelin Receptor A.

Target Information

This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Long-Term Effects of Prenatal Hypoxia on Coronary Artery Function of the Male and Female Offspring.

Biomedicines

Hula N,Liu R,Spaans F,Pasha M,Quon A,Kirschenman R,Cooke CM,Davidge ST

Published figure using Endothelin A Receptor polyclonal antibody (Product # PA3-065) in Immunohistochemistry (Frozen)

Wed Nov 23 00:00:00 EST 2022

Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.

Oncology reports

Torres MJ,López-Moncada F,Herrera D,Indo S,Lefian A,Llanos P,Tapia J,Castellón EA,Contreras HR

Published figure using Endothelin A Receptor polyclonal antibody (Product # PA3-065) in Immunocytochemistry

Sun Aug 01 00:00:00 EDT 2021

Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.

Oncology reports

Torres MJ,López-Moncada F,Herrera D,Indo S,Lefian A,Llanos P,Tapia J,Castellón EA,Contreras HR

Published figure using Endothelin A Receptor polyclonal antibody (Product # PA3-065) in Immunocytochemistry

Sun Aug 01 00:00:00 EDT 2021

Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.

Oncology reports

Torres MJ,López-Moncada F,Herrera D,Indo S,Lefian A,Llanos P,Tapia J,Castellón EA,Contreras HR

Published figure using Endothelin A Receptor polyclonal antibody (Product # PA3-065) in Immunocytochemistry

Sun Aug 01 00:00:00 EDT 2021

Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.

Oncology reports

Torres MJ,López-Moncada F,Herrera D,Indo S,Lefian A,Llanos P,Tapia J,Castellón EA,Contreras HR

Published figure using Endothelin A Receptor polyclonal antibody (Product # PA3-065) in Immunocytochemistry

Sun Aug 01 00:00:00 EDT 2021

Peroxisome proliferator-activated receptor gamma blunts endothelin-1-mediated contraction of the uterine artery in a murine model of high-altitude pregnancy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Lane SL,Doyle AS,Bales ES,Houck JA,Lorca RA,Moore LG,Julian CG

PA3-065 was used in Immunoelectrophoresis to show fetal growth under hypoxic conditions can be improved by pharmacologic PPAR-γ activation.

Sun Mar 01 00:00:00 EST 2020

Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8.

Peptides

Lupp A,Mann A,Heeb A,Kaemmerer D,Sänger J,Evert M,Evert K,Mawrin C,Schulz S

PA3-065 was used in immunohistochemistry - paraffin section to characterize a monoclonal rabbit anti-ETA antibody UMB-8 in and test in sarcomas and in glioblastomas

Wed Apr 01 00:00:00 EDT 2015

Transforming growth factor-β regulates endothelin-1 signaling in the newborn mouse lung during hypoxia exposure.

American journal of physiology. Lung cellular and molecular physiology

Olave N,Nicola T,Zhang W,Bulger A,James M,Oparil S,Chen YF,Ambalavanan N

PA3-065 was used in western blot to investigate the influence of tumor growth factor beta on endothelin-1 expression and effects of hypoxia on the newborn lung

Tue May 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Endothelin A; endothelin A receptor; endothelin receptor subtype A; Endothelin receptor type A; Endothelin-1 receptor; endothelin-1-specific receptor; ET-A; G protein-coupled receptor; G-protein coupled receptor 10

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Gene Aliases: EDNRA; Endor; ET-A; ET-AR; ETA; ETA-R; ETAR; ETRA; Gpcr10; hET-AR; MFDA; RGD1559432

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UniProt ID: (Human) P25101, (Rat) P26684, (Mouse) Q61614

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Entrez Gene ID: (Human) 1909, (Rat) 24326, (Mouse) 13617

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Function(s)

Process(es)

phosphatidylinositol phospholipase C activity endothelin receptor activity protein binding protein tyrosine kinase activity signal transducer activity G-protein coupled receptor activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Endothelin A Receptor Polyclonal Antibody

Endothelin A Receptor Antibody (PA3-065) in WB

Product Details

PA3-065

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoelectrophoresis (IE)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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