Invitrogen

Endothelin B Receptor Polyclonal Antibody

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Antibody Testing Data (4)

Endothelin B Receptor Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Endothelin B Receptor Antibody (PA3-066) in ICC/IF

Immnoctyochemistry of ETB in ETB-transfected cells

Endothelin B Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F))

Endothelin B Receptor Antibody in Immunohistochemistry (IHC)

Endothelin B Receptor Antibody in Western Blot (WB)

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Product Details

PA3-066

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000

Immunohistochemistry (Frozen) (IHC (F))

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:5,000

View 1 publication 1 publication

Immunoelectrophoresis (IE)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

COOH-terminal amino acids of mouse ETB (424-442) KANDHGYDNFRSSNKYSSS coupled to KLH

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2540493

Product Specific Information

PA3-066 detects Endothelin Receptor B from human, mouse, and rat samples.

PA3-066 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects a 47 kDa protein corresponding to mouse Endothelin Receptor B.

The PA3-066 antigen is the COOH-terminal amino acids of mouse ETB (424-442) KANDHGYDNFRSSNKYSSS coupled to KLH.

Target Information

Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1.

Autophagy

Lee B,Shin H,Oh JE,Park J,Park M,Yang SC,Jun JH,Hong SH,Song H,Lim HJ

PA3-066 was used in Western Blot to investigate uterine autophagy in reproductive cycle and pregnancy vascular changes.

Thu Jul 01 00:00:00 EDT 2021

Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.

Oncology reports

Torres MJ,López-Moncada F,Herrera D,Indo S,Lefian A,Llanos P,Tapia J,Castellón EA,Contreras HR

PA3-066 was used in Western Blot to investigate whether levels of endothelin‚1 correspond to castration‚resistant prostate cancer-specific steroidogenesis enzymes, androgen receptor, and testosterone production.

Sun Aug 01 00:00:00 EDT 2021

Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.

Oncology reports

Torres MJ,López-Moncada F,Herrera D,Indo S,Lefian A,Llanos P,Tapia J,Castellón EA,Contreras HR

Sun Aug 01 00:00:00 EDT 2021

Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.

Oncology reports

Torres MJ,López-Moncada F,Herrera D,Indo S,Lefian A,Llanos P,Tapia J,Castellón EA,Contreras HR

Sun Aug 01 00:00:00 EDT 2021

Peroxisome proliferator-activated receptor gamma blunts endothelin-1-mediated contraction of the uterine artery in a murine model of high-altitude pregnancy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Lane SL,Doyle AS,Bales ES,Houck JA,Lorca RA,Moore LG,Julian CG

PA3-066 was used in Immunoelectrophoresis to show fetal growth under hypoxic conditions can be improved by pharmacologic PPAR-γ activation.

Sun Mar 01 00:00:00 EST 2020

Peroxisome proliferator-activated receptor gamma blunts endothelin-1-mediated contraction of the uterine artery in a murine model of high-altitude pregnancy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Lane SL,Doyle AS,Bales ES,Houck JA,Lorca RA,Moore LG,Julian CG

PA3-066 was used in Immunoelectrophoresis to show fetal growth under hypoxic conditions can be improved by pharmacologic PPAR-γ activation.

Sun Mar 01 00:00:00 EST 2020

Transforming growth factor-β regulates endothelin-1 signaling in the newborn mouse lung during hypoxia exposure.

American journal of physiology. Lung cellular and molecular physiology

Olave N,Nicola T,Zhang W,Bulger A,James M,Oparil S,Chen YF,Ambalavanan N

PA3-066 was used in western blot to investigate the influence of tumor growth factor beta on endothelin-1 expression and effects of hypoxia on the newborn lung

Tue May 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: AU022549; endothelin B receptor; Endothelin receptor non-selective type; endothelin receptor subtype B1; Endothelin receptor type B; endothelin-B receptor; ET-B; Et-b receptor; Et-b-r; ET-BR; ET-RB; Etb receptor; Etb-svr; piebald; RP11-318G21.1

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Gene Aliases: ABCDS; Ednra; EDNRB; ET-B; ET-BR; ETB; ETB1; ETBR; ETR-b; ETRB; HSCR; HSCR2; Sox10m1; WS4A

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UniProt ID: (Human) P24530, (Rat) P21451, (Mouse) P48302

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Entrez Gene ID: (Human) 1910, (Rat) 50672, (Mouse) 13618

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Function(s)

endothelin receptor activity protein binding peptide hormone binding type 1 angiotensin receptor binding receptor activity G-protein coupled receptor activity signal transducer activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter neural crest cell migration positive regulation of protein phosphorylation regulation of pH G-protein coupled receptor signaling pathway phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration peripheral nervous system development posterior midgut development aging regulation of blood pressure positive regulation of cell proliferation negative regulation of neuron maturation response to organic cyclic compound vein smooth muscle contraction sensory perception of pain cGMP-mediated signaling melanocyte differentiation regulation of fever generation negative regulation of cellular protein metabolic process response to lipopolysaccharide enteric smooth muscle cell differentiation positive regulation of urine volume positive regulation of renal sodium excretion epithelial fluid transport vasoconstriction vasodilation negative regulation of apoptotic process pigmentation developmental pigmentation macrophage chemotaxis response to pain enteric nervous system development regulation of epithelial cell proliferation regulation of sensory perception of pain positive regulation of penile erection cellular response to lipopolysaccharide endothelin receptor signaling pathway cell surface receptor signaling pathway negative regulation of adenylate cyclase activity nervous system development signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Endothelin B Receptor Polyclonal Antibody

Endothelin B Receptor Antibody (PA3-066) in ICC/IF

Product Details

PA3-066

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoelectrophoresis (IE)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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