Immunofluorescent analysis of EphB2 in A375 cells using an EphB2 polyclonal antibody (Product # PA1-30297) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red) and DAPI was used to stain the nuclei (blue).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH, corresponding to amino acids 1036-1051 of Human Eph B2.|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30297 detects EphB2 from human samples.
PA1-30297 has been successfully used in immunohistochemistry (paraffin tissue), Western blot, and ELISA applications. Suggested positive controls for this produt is NCI-H460 cell lysate and breast carcinoma.
The PA1-30297 immunogen is a synthetic peptide conjugated to KLH, corresponding to amino acids 1036-1051 of Human Eph B2.
Store at 4°C short term. For extended storage aliquot and store at -20°C, avoiding freeze/thaw cycles.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.