|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Purified recombinant mouse EphB3 extracellular domain expressed mouse NSO cells.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28013 detects EphB3 from mouse samples. Expected to cross-react with human due to sequence homology, 96.8%.
PA1-28013 has been successfully used in Western blot and ELISA applications.
The PA1-28013 immunogen is purified recombinant mouse EphB3 extracellular domain expressed mouse NSO cells.
Store at 4°C short term. For long term storage, aliquot and store at -20°C. Avoid freeze-thaw cycles.
EphB3, also known as Cek10, Hek2, Mdk5, Tyro6, and Sek4, is a member of the Eph receptor family, which binds members of the ephrin ligand family. The ephrin ligands and Eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Cell surface receptor mediated signal transduction Receptor protein tyrosine kinase signaling pathway Developmental processes Ectoderm development Neurogenesis Mesoderm development Cell proliferation and differentiation