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Immunofluorescent analysis of Ephrin-A1 was performed using 90% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ephrin-A1 Rabbit Polyclonal Antibody (34-3300) at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal end of mouse ephrin-A1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a member of the ephrin family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||Not Cited||The proangiogenic factor ephrin-A1 is up-regulated in radioresistant murine tumor by irradiation.||Nojiri K,Iwakawa M,Ichikawa Y,Imadome K,Sakai M,Nakawatari M,Ishikawa K,Ishikawa A,Togo S,Tsujii H,Shimada H,Imai T||Experimental biology and medicine (Maywood, N.J.) (234:112)||2009|
|Human||Not Cited||Ephrin-A1 binding to CD4+ T lymphocytes stimulates migration and induces tyrosine phosphorylation of PYK2.||Aasheim HC,Delabie J,Finne EF||Blood (105:2869)||2005|
alpha-induced protein 4; B61; ECKLG; EFL1; EPH-related receptor tyrosine kinase ligand 1; ephrin-A1; EPLG1; immediate early response protein B61; LERK-1; LERK1; ligand of eph-related kinase 1; TNF alpha-induced protein 4; TNFAIP4; tumor necrosis factor; tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4
AI325262; B61; ECKLG; EFL1; EFNA1; Epgl1; Epl1; EPLG1; LERK-1; LERK1; TNFAIP4