|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal sequence of the mouse ephrin-B3 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ephrin-B3 coordinates timed axon targeting and amygdala spinogenesis for innate fear behaviour.
34-3600 was used in western blot to determine how ephrin-B3 coordinates amygdala spinogenesis and timed axon targeting for innate fear behavior
|Zhu XN,Liu XD,Sun S,Zhuang H,Yang JY,Henkemeyer M,Xu NJ||Nature communications (7:null)||2016|
|Mouse||Not Cited||Methylmercury alters Eph and ephrin expression during neuronal differentiation of P19 embryonal carcinoma cells.||Wilson DT,Polunas MA,Zhou R,Halladay AK,Lowndes HE,Reuhl KR||Neurotoxicology (26:661)||2005|
EphA4-dependent axon retraction and midline localization of Ephrin-B3 are disrupted in the spinal cord of mice lacking mDia1 and mDia3 in combination.
34-3600 was used in immunohistochemistry - paraffin section to characterize mice lacking mDia and mDia3.
|Toyoda Y,Shinohara R,Thumkeo D,Kamijo H,Nishimaru H,Hioki H,Kaneko T,Ishizaki T,Furuyashiki T,Narumiya S||Genes to cells : devoted to molecular and cellular mechanisms (18:873)||2013|
Evaluation of Eph receptor and ephrin expression within the human cornea and limbus.
34-3600 was used in immunohistochemistry to study Eph/ephrin expression within the human cornea.
|Hogerheyde TA,Stephenson SA,Harkin DG,Bray LJ,Madden PW,Woolf MI,Richardson NA||Experimental eye research (107:110)||2013|