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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 840-896 of the C-terminal portion of human Eps15. [Swiss-Prot# P42566]|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.04% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunoprecipitation (IP)||5-10 µg/1 mg lysate|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for EPS15 (transient overexpression lysate), Hela whole cell extract.
EPS15 is a ubiquitously expressed protein that is involved in cell growth regulation and may be involved in the regulation of mitogenic signals and control of cell proliferation. Phosphorylation of EPS15 on Tyrosine 849 upon DNA damage is involved in the internalization of EGFR. A chromosomal aberration involving EPS15 is also found in acute leukemias, resulting in a rogue activator protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AF-1p protein; AF1P; ALL1 fused gene from chromosome 1; epidermal growth factor pathway substrate 15; epidermal growth factor receptor substrate 15; EPS15; protein AF-1p
2410112D09Rik; AF-1P; AF1P; EPS15; MLLT5; RGD1307641