Invitrogen

Equine IL-4 Recombinant Protein

Product Details

REIL4I

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	-
ELISA (ELISA)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species	Horse
Published species	Not Applicable
Expression System	Yeast
Amino acid sequence	Equine IL-4, amino acids 24-132 (Accession # P42202)
Molecular weight	12.3 kDa
Class	Recombinant
Type	Protein
Activity	ED50 = 20 - 50 ng/mL; determined by the dose-dependent proliferation of TF-1 cells.
Conjugate	Unconjugated
Form	Lyophilized
Purification	purified
Storage buffer	PBS
Contains	no preservative
Storage conditions	4° C

Product Specific Information

Reconstitute with 0.5 mL of distilled or deionized water. For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.

The biological activity of recombinant equine IL-4 was determined by measuring the proliferation of TF-1 cells. The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 20 - 50 ng/mL. Ref: Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.

Mature equine IL-4 was expressed in Pichia pastoris, X-33. GenBank® Accession # P42202, amino acids 24 - 132. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: CKYDITLQEI.

Reconstitute with 0.5 mL of distilled water.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dendritic Cells Generated From Mops condylurus, a Likely Filovirus Reservoir Host, Are Susceptible to and Activated by Zaire Ebolavirus Infection.

Frontiers in immunology

Edenborough KM,Bokelmann M,Lander A,Couacy-Hymann E,Lechner J,Drechsel O,Renard BY,Radonić A,Feldmann H,Kurth A,Prescott J

Mon Nov 09 00:00:00 EST 2020

Bioinformatics

Protein Aliases: B-cell stimulatory factor 1; BSF-1; H-IL-4; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

Gene Aliases: BSF-1; IL-4; IL4

UniProt ID: (Horse) P42202

Entrez Gene ID: (Horse) 100034225

Molecular Function: interleukin superfamily

Equine IL-4 Recombinant Protein

Product Details

REIL4I

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Activity

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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