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Invitrogen
ErbB3 Polyclonal Antibody
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FIGURE: 1 / 4
ErbB3 Antibody (PA1-86644) in ICC/IF
Immunofluorescence analysis of ErbB3 was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ErbB3 Goat Polyclonal Antibody (Product # PA1-86644) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at ... View More
Product Details
PA1-86644
Applications
Tested Dilution
Publications
Product Specifications
Published species
Dog
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
peptide sequence C-HSRLFPKANAQRT corresponding to the C-terminal of ErbB3 isoform 1(NP_001973.2).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Storage conditions
4°C or -20°C if preferred
Shipping conditions
Wet ice
RRID
Product Specific Information
Predicted to react with dog, mouse and rat based on sequence homology.
In Western blot, this antibody detects a band at ~150 kDa in Jurkat cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
Goat anti Human Erb3 antibody recognizes the human Receptor tyrosine-protein kinase erbB-3, also known as ErbB3, Proto-oncogene-like protein c-ErbB-3 or Tyrosine kinase-type cell surface receptor HER3.
Target Information
ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family. ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6. 2kb mRNA which is translated into a 160/180 kDa glycoprotein. The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity. Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation. Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized. One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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