|Tested species reactivity||Human|
|Published species reactivity||Dog|
|Host / Isotype||Goat / IgG|
|Immunogen||peptide sequence C-HSRLFPKANAQRT corresponding to the C-terminal of ErbB3 isoform 1(NP_001973.2).|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1 - 0.3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
Predicted to react with dog, mouse and rat based on sequence homology.
In Western blot, this antibody detects a band at ~150 kDa in Jurkat cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
High basal Wnt signaling is further induced by PI3K/mTor inhibition but sensitive to cSRC inhibition in mammary carcinoma cell lines with HER2/3 overexpression.
PA1-86644 was used in western blot to assess the expression and function of EGFR/HER-regulated pathways on the ligand-independent Wnt signaling in canine breast cancer
|Timmermans-Sprang EP,Gracanin A,Mol JA||BMC cancer (15:null)||2015|