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Invitrogen
ErbB3 Polyclonal Antibody
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FIGURE: 1 / 2
ErbB3 Antibody (PA5-87529) in WB
Western blot analysis of ErbB3 in extracts of various cell lines. Samples were incubated with ErbB3 Polyclonal antibody (Product # PA5-87529) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-87529
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1275-1342 of human ErbB3/HER3 (NP_0019732)
Conjugate
Form
Liquid
Concentration
2.31 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: DYAAMGACPA SEQGYEEMRA FQGPGHQAPH VHYARLKTLR SLEATDSAFD NPDYWHSRLF PKANAQRT
Positive Samples: Mouse skeletal muscle, Mouse lung, Rat skeletal muscle; Cellular Location: Cell membrane, Single-pass type I membrane protein
Target Information
ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family. ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6. 2kb mRNA which is translated into a 160/180 kDa glycoprotein. The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity. Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation. Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized. One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: avian erythroblastosis oncogene B 3 receptor; c-erbB-3; C-errB-3; Glial growth factor receptor; human epidermal growth factor receptor 3; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine kinase; Receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
Gene Aliases: c-erbB-3; c-erbB3; C76256; ErbB-3; ERBB3; erbB3-S; Erbb3r; HER3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3
UniProt ID: (Human) P21860, (Mouse) Q61526
Entrez Gene ID: (Human) 2065, (Mouse) 13867
protein tyrosine kinase activity
transmembrane signaling receptor activity
Ras guanyl-nucleotide exchange factor activity
protein binding
ATP binding
growth factor binding
protein tyrosine kinase activator activity
neuregulin binding
identical protein binding
protein homodimerization activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
protein heterodimerization activity
nucleotide binding
protein kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
neuregulin receptor activity
transmembrane signal receptor
MAPK cascade
endocardial cushion development
negative regulation of cell adhesion
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
peripheral nervous system development
heart development
negative regulation of signal transduction
Schwann cell differentiation
phosphatidylinositol 3-kinase signaling
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cranial nerve development
ERBB2 signaling pathway
wound healing
regulation of cell proliferation
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
negative regulation of secretion
neuron apoptotic process
positive regulation of protein tyrosine kinase activity
positive regulation of calcineurin-NFAT signaling cascade
extrinsic apoptotic signaling pathway in absence of ligand
regulation of cell motility
protein phosphorylation
epidermal growth factor receptor signaling pathway
phosphorylation
positive regulation of glucose import
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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