Invitrogen

ErbB4 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (49) ErbB4 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)
Advanced Verification (1)

ErbB4 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 4

ErbB4 Antibody (PA5-82452) in IHC

Immunohistochemical analysis of ErbB4 in human testis and liver tissues using a ErbB4 polyclonal antibody (Product # PA5-82452). Corresponding RNA-seq data are presented for the same tissues.

Product Image Gallery

ErbB4 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of ErbB4 demonstrates antibody specificity. Immunohistochemical analysis of ErbB4 using anti-ErbB4 Polyclonal Antibody (Product #PA5-82452), shows significant staining of ErbB4 in testis and shows minimal or weak staining in liver tissues. The relative expression levels of ErbB4 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-82452

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ERBB4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Anitgen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2789610

Product Specific Information

Immunogen sequence: LPSPNDSKFF QNLLDEEDLE DMMDAEEYLV PQAFNIPPPI YTSRARIDSN RNQFVYRDGG FAAEQGVSVP YRAPTSTIPE APVAQGATAE IFDDSCCNGT LRK

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 4ICD; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; ERBB4 intracellular domain; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; s80HER4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

Gene Aliases: ALS19; ERBB4; HER4; p180erbB4

UniProt ID: (Human) Q15303

Entrez Gene ID: (Human) 2066

Molecular Function: transmembrane signal receptor

Function(s)

Process(es)

protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding protein binding ATP binding protein homodimerization activity transcription regulatory region DNA binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity