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Invitrogen
ErbB4 Polyclonal Antibody
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FIGURE: 1 / 1
ErbB4 Antibody (PA5-87501) in WB
Western blot analysis of ErbB4 in extracts of Rat brain. Samples were incubated with ErbB4 Polyclonal antibody (Product # PA5-87501) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-87501
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1150-1250 of human ErbB4/HER4 (NP_0052261)
Conjugate
Form
Liquid
Concentration
1.45 mg/mL
Amount
145 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: YMTPMRDKPK QEYLNPVEEN PFVSRRKNGD LQALDNPEYH NASNGPPKAE DEYVNEPLYL NTFANTLGKA EYLKNNILSM PEKAKKAFDN PDYWNHSLPP R; Positive Samples: Rat brain; Cellular Location: Cell membrane, Mitochondrion, Nucleus, Single-pass type I membrane protein
Target Information
Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-erbB-4; OTTHUMP00000209432; Proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; Receptor tyrosine-protein kinase erbB-4; v-erb-a erythroblastic leukemia viral oncogene homolog 4
Gene Aliases: Erbb4; Tyro-2
UniProt ID: (Rat) Q62956
Entrez Gene ID: (Rat) 59323
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
epidermal growth factor receptor binding
protein binding
ATP binding
protein homodimerization activity
transcription regulatory region DNA binding
transmembrane signal receptor
neural crest cell migration
transcription, DNA-templated
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
nervous system development
heart development
lactation
positive regulation of cell proliferation
negative regulation of cell proliferation
embryonic pattern specification
negative regulation of muscle cell apoptotic process
cell migration
peptidyl-tyrosine phosphorylation
telencephalon development
central nervous system morphogenesis
olfactory bulb interneuron differentiation
signal transduction by protein phosphorylation
regulation of cell migration
positive regulation of cell migration
mammary gland development
positive regulation of synaptic transmission, GABAergic
response to progesterone
odontogenesis
response to drug
positive regulation of tyrosine phosphorylation of Stat5 protein
response to hydrogen peroxide
negative regulation of apoptotic process
surfactant homeostasis
positive regulation of phosphatidylinositol 3-kinase activity
mitochondrial fragmentation involved in apoptotic process
cell fate commitment
positive regulation of transcription, DNA-templated
positive regulation of glucose import
protein autophosphorylation
positive regulation of epithelial cell proliferation
positive regulation of cardiac muscle cell proliferation
synapse maturation
mammary gland epithelial cell differentiation
mammary gland alveolus development
cardiac muscle tissue regeneration
positive regulation of ERK1 and ERK2 cascade
positive regulation of STAT protein import into nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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