Invitrogen

ErbB4 Polyclonal Antibody

View all (51) ErbB4 antibodies

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Product Details

PA5-95075

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 4 (35-51aa KLSSLSDLEQQYRALRK).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806881

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: A549 Cell, HELA Cell, HELA Cell, U87 Cell, NEURO Cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; c-erbB-4; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; OTTHUMP00000209432; p180erbB4; Proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; Receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

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Gene Aliases: ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4; Tyro-2

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UniProt ID: (Human) Q15303, (Mouse) Q61527, (Rat) Q62956

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Entrez Gene ID: (Human) 2066, (Mouse) 13869, (Rat) 59323

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Function(s)
protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding protein binding ATP binding protein homodimerization activity transcription regulatory region DNA binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor
Process(es)
MAPK cascade neural crest cell migration positive regulation of protein phosphorylation transcription, DNA-templated signal transduction transmembrane receptor protein tyrosine kinase signaling pathway nervous system development heart development lactation cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation embryonic pattern specification regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling cell migration peptidyl-tyrosine phosphorylation central nervous system morphogenesis olfactory bulb interneuron differentiation regulation of cell migration ERBB2 signaling pathway positive regulation of tyrosine phosphorylation of Stat5 protein positive regulation of GTPase activity mitochondrial fragmentation involved in apoptotic process cell fate commitment positive regulation of transcription, DNA-templated protein autophosphorylation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of cardiac muscle cell proliferation mammary gland epithelial cell differentiation mammary gland alveolus development cardiac muscle tissue regeneration positive regulation of ERK1 and ERK2 cascade positive regulation of protein localization to cell surface regulation of cell motility positive regulation of STAT protein import into nucleus negative regulation of neuron migration regulation of transcription, DNA-templated protein phosphorylation apoptotic process multicellular organism development negative regulation of muscle cell apoptotic process phosphorylation positive regulation of cell migration positive regulation of synaptic transmission, GABAergic negative regulation of apoptotic process surfactant homeostasis positive regulation of glucose import positive regulation of epithelial cell proliferation synapse maturation telencephalon development signal transduction by protein phosphorylation mammary gland development response to progesterone odontogenesis response to drug response to hydrogen peroxide positive regulation of phosphatidylinositol 3-kinase activity
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