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          • Primary Antibodies ›
          • ERK1/2 Antibodies

          Rockland

          Erk1/2 Polyclonal Antibody

          View all (82) ERK1/2 antibodies

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          Cite Erk1/2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          Erk1/2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Erk1/2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Erk1/2 Antibody (100-401-F25) in ICC/IF

          Immunofluorescence Microscopy of Rabbit anti-ERK1/2 antibody. Tissue: backskin sections of transgenic mice. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: ERK1/2 antibody at 10 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ERK1/2 is cytoplasmic. Staining: ERK1/2 as green fluorescent signal. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Erk1/2 Antibody in Immunocytochemistry (ICC/IF)
          Erk1/2 Antibody in Western Blot (WB)

          Product Details

          100-401-F25

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:5,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50
          -

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Chicken, Fruit fly, Human, Mouse, Sheep, Rat, Xenopus

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Erk 1/2 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the synthetic peptide coupled to KLH.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 50% glycerol

          Contains

          0.09% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Bioinformatics

          Protein Aliases: CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; Diphospho-ERK; dp-ERK; dpERK; drosophila ERK; enhancer of seven in absentia 7; ERK; Erk MAP kinase; ERK-1; ERK-2; ERK/rolled; erk1 erk2; Erk1; cancer susceptibility protein; ERK1b; ERK1c; Erk2; cancer susceptibility protein; ERT1; ERT2; extracellular signal-regulated kinase; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; Insulin-stimulated MAP2 kinase; M phase MAP kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAP-kinase; MAPK; MAPK 1; MAPK 2; MAPK 3; MBP kinase; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 1a; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; Myelin basic protein kinase; Myelin xP42 protein kinase; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; phospho-ERK; pp42/MAP kinase; Protein rolled; protein tyrosine kinase ERK2; RE08694p; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; sevenmaker; unnamed protein product

          View more View less

          Gene Aliases: 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG18732; CT34260; CT39192; D-ERK; DERK; DERK-A; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmERK; DmERK-A; DmERKA; DmMAPK; dp-ERK; DpERK; dpERK1; dpMAPK; Dsor2; E(sina)7; EC2-1; EK2-1; ERK; ERK-1; ERK-2; ERK-A; Erk/Map kinase; ERK1; ERK1/2; ERK2; ERKA; ERT1; ERT2; Esrk1; EY2-2; GroupII; HS44KDAP; HUMKER1A; l(2)41Ac; l(2R)EMS45-39; MAP-k; MAPK; MAPK1; mapk1-a; mapk1-b; mapk1a; MAPK2; MAPK3; MNK1; mpk1; Mtap2k; NS13; p-ERK; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; pERK; pMAPK; PRKM1; PRKM2; PRKM3; RL; rl/ERK; rl/MAPK; rll; SEM; SR2-1; Su(Raf)2B; XELAEV_18010534mg; xp42

          View more View less

          UniProt ID: (Bovine) P46196, (Human) P28482, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708, (Xenopus) P26696, (Fruit fly) P40417

          View more View less

          Entrez Gene ID: (Bovine) 327672, (Human) 5594, (Sheep) 101115485, (Bovine) 531391, (Chicken) 373953, (Sheep) 101123008, (Human) 5595, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689, (Xenopus) 398985, (Fruit fly) 3354888

          View more View less

          Function(s)
          phosphotyrosine binding protein serine/threonine kinase activity MAP kinase kinase activity ATP binding RNA polymerase II carboxy-terminal domain kinase activity phosphatase binding identical protein binding nucleotide binding DNA binding protein kinase activity MAP kinase activity protein binding kinase activity transferase activity protein serine kinase activity DNA-binding transcription factor binding transcription factor binding protein kinase binding mitogen-activated protein kinase kinase kinase binding transferase activity, transferring phosphorus-containing groups scaffold protein binding non-receptor serine/threonine protein kinase
          Process(es)
          activation of MAPK activity protein phosphorylation apoptotic process cellular response to DNA damage stimulus cell cycle positive regulation of gene expression positive regulation of peptidyl-threonine phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytosine metabolic process regulation of ossification regulation of cellular pH thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase mammary gland epithelial cell proliferation cellular response to amino acid starvation cellular response to reactive oxygen species response to nicotine ERBB signaling pathway outer ear morphogenesis response to exogenous dsRNA negative regulation of cell differentiation thymus development T cell receptor signaling pathway B cell receptor signaling pathway stress-activated MAPK cascade positive regulation of telomerase activity Bergmann glial cell differentiation long-term synaptic potentiation face development lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade response to epidermal growth factor cellular response to cadmium ion cellular response to tumor necrosis factor cellular response to granulocyte macrophage colony-stimulating factor stimulus cellular response to dopamine positive regulation of telomere capping MAPK cascade regulation of transcription from RNA polymerase II promoter chemotaxis signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway chemical synaptic transmission heart development learning or memory insulin receptor signaling pathway organ morphogenesis positive regulation of macrophage chemotaxis neural crest cell development Schwann cell development positive regulation of telomere maintenance response to lipopolysaccharide regulation of stress-activated MAPK cascade intracellular signal transduction ERBB2-ERBB3 signaling pathway myelination positive regulation of cholesterol biosynthetic process insulin-like growth factor receptor signaling pathway regulation of cytoskeleton organization interleukin-34-mediated signaling pathway chemokine-mediated signaling pathway caveolin-mediated endocytosis regulation of Golgi inheritance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response regulation of early endosome to late endosome transport autophagosome assembly negative regulation of T cell mediated immune response to tumor cell transcription, DNA-templated regulation of gene expression negative regulation of autophagy positive regulation of autophagy phosphorylation sensory perception of pain BMP signaling pathway positive regulation of cyclase activity cellular response to nutrient levels negative regulation of proteasomal ubiquitin-dependent protein catabolic process peptidyl-tyrosine autophosphorylation TORC1 signaling glucose homeostasis signal transduction in response to DNA damage positive regulation of transcription from RNA polymerase II promoter modulation of synaptic transmission protein stabilization negative regulation of T cell activation cartilage development positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus negative regulation of cholesterol efflux xenophagy negative regulation of TORC1 signaling positive regulation of xenophagy MAPK import into nucleus positive regulation of cell proliferation response to toxic substance positive regulation of cell migration response to estrogen positive regulation of translation positive regulation of transcription, DNA-templated cellular response to organic substance regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein phosphorylation protein complex assembly DNA damage induced protein phosphorylation positive regulation of histone phosphorylation positive regulation of histone acetylation negative regulation of apolipoprotein binding arachidonic acid metabolic process mitotic cell cycle terminal region determination terminal branching, open tracheal system R7 cell fate commitment imaginal disc-derived wing vein specification imaginal disc-derived wing morphogenesis leg disc proximal/distal pattern formation metamorphosis torso signaling pathway determination of adult lifespan fibroblast growth factor receptor signaling pathway cellular response to starvation negative regulation of macroautophagy lymph gland plasmatocyte differentiation lymph gland crystal cell differentiation tracheal pit formation in open tracheal system sevenless signaling pathway positive regulation of cell size positive regulation of photoreceptor cell differentiation dorsal appendage formation vascular endothelial growth factor receptor signaling pathway behavioral response to ethanol positive regulation of sequence-specific DNA binding transcription factor activity defense response to virus branching involved in open tracheal system development cellular response to arsenic-containing substance positive regulation of wound healing regulation of heart morphogenesis regulation of response to drug
          It has to be done as per old AB suggested Products section.

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