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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ERK1/2 Antibodies

          Rockland

          Erk1/2 Polyclonal Antibody

          View all (100) ERK1/2 antibodies

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          Datasheet
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          Datasheet
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          Cite Erk1/2 Polyclonal Antibody

          • Antibody Testing Data (2)
          Erk1/2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Erk1/2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Erk1/2 Antibody (100-401-F25) in ICC/IF

          Immunofluorescence Microscopy of Rabbit anti-ERK1/2 antibody. Tissue: backskin sections of transgenic mice. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: ERK1/2 antibody at 10 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ERK1/2 is cytoplasmic. Staining: ERK1/2 as green fluorescent signal. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Erk1/2 Antibody in Immunocytochemistry (ICC/IF)
          Erk1/2 Antibody in Western Blot (WB)

          Product Details

          100-401-F25

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:5,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50
          -

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Chicken, Fruit fly, Human, Mouse, Sheep, Rat, Xenopus

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Erk 1/2 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the synthetic peptide coupled to KLH.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 50% glycerol

          Contains

          0.09% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; Diphospho-ERK; dp-ERK; dpERK; drosophila ERK; enhancer of seven in absentia 7; ERK; Erk MAP kinase; ERK-1; ERK-2; ERK/rolled; erk1 erk2; ERK1b; ERT1; ERT2; extracellular signal-regulated kinase; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; Insulin-stimulated MAP2 kinase; M phase MAP kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAP-kinase; MAPK; MAPK 1; MAPK 2; MAPK 3; MBP kinase; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 1a; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; Myelin basic protein kinase; Myelin xP42 protein kinase; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; phospho-ERK; pp42/MAP kinase; Protein rolled; protein tyrosine kinase ERK2; RE08694p; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; sevenmaker

          View more View less

          Gene Aliases: 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG18732; CT34260; CT39192; D-ERK; DERK; DERK-A; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmERK; DmERK-A; DmERKA; DmMAPK; dp-ERK; DpERK; dpERK1; dpMAPK; Dsor2; E(sina)7; EC2-1; EK2-1; ERK; ERK-1; ERK-2; ERK-A; Erk/Map kinase; ERK1; ERK1/2; ERK2; ERKA; ERT1; ERT2; Esrk1; EY2-2; GroupII; HS44KDAP; HUMKER1A; l(2)41Ac; l(2R)EMS45-39; MAP-k; MAPK; MAPK1; mapk1-a; mapk1-b; mapk1a; MAPK2; MAPK3; MNK1; mpk1; Mtap2k; p-ERK; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; pERK; pMAPK; PRKM1; PRKM2; PRKM3; RL; rl/ERK; rl/MAPK; rll; SEM; SR2-1; Su(Raf)2B; XELAEV_18010534mg; xp42

          View more View less

          UniProt ID: (Human) P28482, (Bovine) P46196, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708, (Xenopus) P26696, (Fruit fly) P40417

          View more View less

          Entrez Gene ID: (Human) 5594, (Bovine) 327672, (Sheep) 101115485, (Bovine) 531391, (Human) 5595, (Chicken) 373953, (Sheep) 101123008, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689, (Xenopus) 398985, (Fruit fly) 3354888

          View more View less

          Function(s)
          phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding MAP kinase kinase activity scaffold protein binding protein kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
          Process(es)
          MAPK cascade activation of MAPK activity MAPK import into nucleus transcription, DNA-templated protein phosphorylation apoptotic process chemotaxis response to stress cellular response to DNA damage stimulus cell cycle signal transduction chemical synaptic transmission axon guidance positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway response to toxic substance positive regulation of peptidyl-threonine phosphorylation regulation of phosphatidylinositol 3-kinase signaling viral process peptidyl-serine phosphorylation peptidyl-threonine phosphorylation sensory perception of pain cytosine metabolic process platelet activation positive regulation of cell migration thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase regulation of stress-activated MAPK cascade mammary gland epithelial cell proliferation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB signaling pathway outer ear morphogenesis response to exogenous dsRNA response to estrogen negative regulation of cell differentiation positive regulation of translation positive regulation of transcription, DNA-templated thymus development T cell receptor signaling pathway B cell receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity regulation of cytoskeleton organization positive regulation of telomerase activity Bergmann glial cell differentiation long-term synaptic potentiation face development JAK-STAT cascade involved in growth hormone signaling pathway lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade response to epidermal growth factor caveolin-mediated endocytosis regulation of Golgi inheritance cellular response to granulocyte macrophage colony-stimulating factor stimulus regulation of cellular response to heat positive regulation of telomere capping regulation of early endosome to late endosome transport positive regulation of gene expression regulation of ossification regulation of cellular pH cellular response to amino acid starvation cellular response to reactive oxygen species response to nicotine stress-activated MAPK cascade cellular response to cadmium ion cellular response to tumor necrosis factor cellular response to dopamine positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly DNA damage induced protein phosphorylation phosphorylation arachidonic acid metabolic process BMP signaling pathway positive regulation of cyclase activity positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation positive regulation of transcription from RNA polymerase II promoter cartilage development positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus positive regulation of xenophagy negative regulation of apolipoprotein binding organ morphogenesis response to lipopolysaccharide intracellular signal transduction cellular response to organic substance mitotic cell cycle cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway terminal region determination terminal branching, open tracheal system R7 cell fate commitment imaginal disc-derived wing vein specification imaginal disc-derived wing morphogenesis leg disc proximal/distal pattern formation metamorphosis insulin receptor signaling pathway torso signaling pathway determination of adult lifespan cellular response to starvation regulation of gene expression negative regulation of macroautophagy lymph gland plasmatocyte differentiation lymph gland crystal cell differentiation tracheal pit formation in open tracheal system sevenless signaling pathway positive regulation of cell size positive regulation of photoreceptor cell differentiation dorsal appendage formation vascular endothelial growth factor receptor signaling pathway behavioral response to ethanol modulation of synaptic transmission positive regulation of sequence-specific DNA binding transcription factor activity defense response to virus branching involved in open tracheal system development cellular response to arsenic-containing substance positive regulation of wound healing regulation of heart morphogenesis regulation of response to drug
          It has to be done as per old AB suggested Products section.
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