Invitrogen

Estrogen Receptor alpha Polyclonal Antibody

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IP-mass spectrometry

2 Published Figures

4 References

View all (115) Estrogen Receptor alpha antibodies

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Estrogen Receptor alpha Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Estrogen Receptor (ER, green) in T47D and Hs 578T breast cancer cells. The cells were fixed with 4% paraformaldehyde for 15 min at room temp, permeabilized with 0.1% Triton X-100 for 15 min at room temp, and blocked with 0.3% BSA in PBS for at least 30 min at room temp. Cells were stained with an ER polyclonal antibody (Product # PA5-16440) at a dilution of 1:100 in blocking buffer overnight at 4C, and then incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:500 for at least 1 hour at room temp. Nuclei (blue) were stained with DAPI (Product # 62247). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Estrogen Receptor alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER, alpha antibody6 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.

Estrogen Receptor alpha Antibody in Western Blot (WB) — Western blot analysis of Estrogen Receptor (ER) was performed by loading 20 µg of whole cell lysates (WCL) or nuclear extracts (NE) from the indicated breast cancer cell lines, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Blotter (Product # 62288), and blocked with 5% milk in TBST for at least 1 hour at room temp. ER was detected at ~43 kD (top panel) using an ER polyclonal antibody (Product # PA5-16440) at a dilution of 1:500 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:40,000. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). To check for equivalent protein loading in each lane, the blot was stripped with Restore Plus Western blot stripping buffer (Product # 46430) for at least 15 min at room temp, re-blocked with 5% milk in TBST for at least 1 hour at room temp, and re-probed with an HDAC1 polyclonal antibody (Product # PA1-860, bottom panel) at a dilution of 1:1000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:40,000 and chemiluminescent detection.The molecular weight of the endogenous protein detected is consistent with isoforms of either ER alpha or ER beta.

Estrogen Receptor alpha Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Estrogen Receptor using Estrogen Receptor Polyclonal Antibody (Product # PA5-16440) on denatured Human MCF-7 Cells.

Estrogen Receptor alpha Antibody — The specificity of anti-ER alpha polyclonal antibody (Product # PA5-16440) was demonstrated in Immunofluorescence analysis by the detection of endogenous Estrogen Receptor Alpha protein in T47D cells, but not in negative control line Hs578T which is not known to express Estrogen Receptor Alpha protein. {RE}

Product Details

PA5-16440

Applications	Tested Dilution	Publications
Western Blot (WB)	2.5-5 µg/mL	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:62.5-1:125	-
Immunocytochemistry (ICC/IF)	1:50-1:200	View 2 publications 2 publications
Immunoprecipitation (IP)	10 µg/mL	-

Product Specifications
Species Reactivity	Hamster, Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide derived from the C-terminus of human estrogen receptor, alpha protein View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10983848

Product Specific Information

PA5-16440 targets Estrogen Receptor in IF, IHC (P), IP, and WB applications and shows reactivity with mouse, Hamster, and Human samples.

The PA5-16440 immunogen is a synthetic peptide derived from the C-terminus of human estrogen receptor, alpha protein.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Continuous Exposure of Breast Cancer Cells to Tamoxifen Upregulates GPER-1 and Increases Cell Proliferation.

Frontiers in endocrinology

Molina L,Bustamante F,Ortloff A,Ramos I,Ehrenfeld P,Figueroa CD

Published figure using Estrogen Receptor alpha polyclonal antibody (Product # PA5-16440) in Western Blot

Fri May 21 00:00:00 EDT 2021

Continuous Exposure of Breast Cancer Cells to Tamoxifen Upregulates GPER-1 and Increases Cell Proliferation.

Frontiers in endocrinology

Molina L,Bustamante F,Ortloff A,Ramos I,Ehrenfeld P,Figueroa CD

Published figure using Estrogen Receptor alpha polyclonal antibody (Product # PA5-16440) in Western Blot

Fri May 21 00:00:00 EDT 2021

Jie-Yu Pill, A Proprietary Herbal Medicine, Ameliorates Mood Disorder-Like Behavior and Cognitive Impairment in Estrogen-Deprived Mice Exposed to Chronic Unpredictable Mild Stress: Implication for a Potential Therapy of Menopause Syndrome.

Frontiers in psychiatry

Zhou XD,Yang XJ,Zheng Y,Qin ZS,Sha W,Chen G,Zhang ZJ

Published figure using Estrogen Receptor alpha polyclonal antibody (Product # PA5-16440) in Western Blot

Fri Dec 18 00:00:00 EST 2020

Frontiers in psychiatry

Zhou XD,Yang XJ,Zheng Y,Qin ZS,Sha W,Chen G,Zhang ZJ

Published figure using Estrogen Receptor alpha polyclonal antibody (Product # PA5-16440) in Western Blot

Fri Dec 18 00:00:00 EST 2020

27-hydroxycholesterol decreases cell proliferation in colon cancer cell lines.

Biochimie

Warns J,Marwarha G,Freking N,Ghribi O

PA5-16440 was used in Immunocytochemistry-immunoflourescence to show for the first time that the cholesterol metabolite 27-OHC reduces cell proliferation in colorectal cancer cells.

Mon Oct 01 00:00:00 EDT 2018

ER-α36-mediated rapid estrogen signaling positively regulates ER-positive breast cancer stem/progenitor cells.

PloS one

Deng H,Zhang XT,Wang ML,Zheng HY,Liu LJ,Wang ZY

PA5-16440 was used in immunocytochemistry and western blot to study the modulation of ER-positive breast cancer stem/progenitior cell growth by ER-alpha36 rapid estrogen signaling

Tue Jan 13 00:00:00 EST 2015

Bioinformatics

Protein Aliases: DKFZp686N23123; ER; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; hER-alpha36; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1

Gene Aliases: ER; ER-alpha; ERa; ERalpha; ESR; ESR1; ESRA; Estr; Estra; ESTRR; I79_001166; NR3A1

UniProt ID: (Human) P03372, (Mouse) P19785

Entrez Gene ID: (Human) 2099, (Mouse) 13982, (Chinese hamster) 100768474

Function(s)

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity steroid binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding nitric-oxide synthase regulator activity estrogen receptor activity type 1 metabotropic glutamate receptor binding protein complex binding estrogen response element binding phosphatidylinositol 3-kinase regulatory subunit binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding hormone binding identical protein binding ATPase binding

Estrogen Receptor alpha Polyclonal Antibody

Product Details

PA5-16440

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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