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Immunofluorescent analysis using ER-beta polyclonal antibody (Product# PA1-310B) shows nuclear localization of ER-beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ER-beta (Product# PA1-310B), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Pig, Hamster, Sheep, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(467) S S T E D S K N K E S S Q N L Q S Q(485) of rat ER beta.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1:100 - 1:1000|
|Immunofluorescence (IF)||1:100 - 1:1000|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:50|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 19 publications below|
|Immunohistochemistry (IHC)||See 19 publications below|
|Gel Shift (GS)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunocytochemistry (ICC)||See 5 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
PA1-310B has been successfully used in Western blot, immunohistochemistry, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing ER beta from both rat brain homogenate as well as various breast cancer cell lines. Immunocytochemical staining of ER beta in cells overexpressing the human protein results in nuclear staining.
The PA1-310B immunogen is a synthetic peptide corresponding to residues C(467) S S T E D S K N K E S S Q N L Q S Q(485) of rat ER beta. This immunizing peptide (PEP-007) is available for use in neutralization and control experiments.
Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.
The Estrogen Receptor beta (ER beta) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. The size and structure of ER beta is very similar to ER alpha, with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as the transactivation domain has diverged significantly. Similar in function to ER alpha, ER beta binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements (ERE's).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Placental STAT3 signaling is activated in women with polycystic ovary syndrome.
PA1-310B was used in western blot to study STAT3 and mTOR signaling in patients with polycystic ovary syndrome
|Maliqueo M,Sundström Poromaa I,Vanky E,Fornes R,Benrick A,Åkerud H,Stridsklev S,Labrie F,Jansson T,Stener-Victorin E||Human reproduction (Oxford, England) (30:692)||2015|
Methoxychlor and its metabolite HPTE inhibit cAMP production and expression of estrogen receptors α and β in the rat granulosa cell in vitro.
PA1-310B was used in western blot to test the effect of methoxychlor/HPTE on FSH-stimulated cAMP production in cultured granulosa cells.
|Harvey CN,Chen JC,Bagnell CA,Uzumcu M||Reproductive toxicology (Elmsford, N.Y.) (51:72)||2015|
Different expression of membrane-bound and cytosolic hippocampal steroid receptor complexes during spatial training in young male rats.
PA1-310B was used in western blot to examine the expression of membrane and cytosolic hippocampal receptors during spatial learning
|Sase S,Meyer K,Lubec G,Korz V||European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology (24:1819)||2014|
Effects of in utero exposure to di(n-butyl) phthalate for estrogen receptors α, β, and androgen receptor of Leydig cell on rats.
PA1-310B was used in immunohistochemistry and western blot to study the expression of ER-alpha, ER-beta and AR in rat Leydig cells following in utero di(n-butyl) phthalate exposure
|Wakui S,Shirai M,Motohashi M,Mutou T,Oyama N,Wempe MF,Takahashi H,Inomata T,Ikegami M,Endou H,Asari M||Toxicologic pathology (42:877)||2014|
Plant sterol feeding induces tumor formation and alters sterol metabolism in the intestine of Apc(Min) mice.
PA1-310B was used in western blot to assess how plant sterol esters alter intestinal tumorigenesis in adenomatous polyposis coli mice
|Marttinen M,Pajari AM,Päivärinta E,Storvik M,Marttinen P,Nurmi T,Niku M,Piironen V,Mutanen M||Nutrition and cancer (66:259)||2014|
Age-dependent effects of prenatal stress on the corticolimbic dopaminergic system development in the rat male offspring.
PA1-310B was used in western blot to study the effects of pre-natal exposure to stress on the development of the corticolimbic dopaminergic system of male offspring in a rat model
|Pallarés ME,Baier CJ,Adrover E,Monteleone MC,Brocco MA,Antonelli MC||Neurochemical research (38:2323)||2013|
Selective oestrogen receptor modulators differentially potentiate brain mitochondrial function.
PA1-310B was used in western blot to investigate the effect of estrogen receptors on mitochondrial function in neurons
|Irwin RW,Yao J,To J,Hamilton RT,Cadenas E,Brinton RD||Journal of neuroendocrinology (24:236)||2012|
In vivo stimulation of oestrogen receptor α increases insulin-stimulated skeletal muscle glucose uptake.
PA1-310B was used in western blot to investgiate the effect of estrogen receptor stimulation on glucose transport in skeletal muscles
|Gorres BK,Bomhoff GL,Morris JK,Geiger PC||The Journal of physiology (589:2041)||2011|
Altered estrogen receptor expression in skeletal muscle and adipose tissue of female rats fed a high-fat diet.
PA1-310B was used in western blot to investigate the effect of dietary fat on estrogen receptor expression
|Gorres BK,Bomhoff GL,Gupte AA,Geiger PC||Journal of applied physiology (Bethesda, Md. : 1985) (110:1046)||2011|
Roles of estrogen receptors alpha and beta in sexually dimorphic neuroprotection against glutamate toxicity.
PA1-310B was used in western blot to investigate the importance of estrogen receptors in the neuroprotective effect against glutamate toxicity
|Bryant DN,Dorsa DM||Neuroscience (170:1261)||2010|
The ability of oestradiol administration to regulate protein levels of oestrogen receptor alpha in the hippocampus and prefrontal cortex of middle-aged rats is altered following long-term ovarian hormone deprivation.
PA1-310B was used in western blot to study the effect of long-term ovarian hormone deprivation on neuronal response to oestradiol administration
|Bohacek J,Daniel JM||Journal of neuroendocrinology (21:640)||2009|
Estrogen receptor alpha (ERalpha) mediates stimulatory effects of estrogen on aromatase (CYP19) gene expression in human placenta.
PA1-310B was used in western blot to study the role of estrogen receptor-alpha in the induction of human placenta aromatase expression by estrogen
|Kumar P,Kamat A,Mendelson CR||Molecular endocrinology (Baltimore, Md.) (23:784)||2009|
Dose-dependent effects of genistein and daidzein on protein metabolism in porcine myotube cultures.
PA1-310B was used in western blot to investigate the effect of isoflavones genistein and daidzein on skeletal muscle cell differentiation and protein metabolism
|Rehfeldt C,Kalbe C,Nürnberg G,Mau M||Journal of agricultural and food chemistry (57:852)||2009|
Estrogen promotes tumor progression in a genetically defined mouse model of lung adenocarcinoma.
PA1-310B was used in western blot to use a genetically defined murine lung adenocarcinoma model to study the effects of estrogen on tumor progression
|Hammoud Z,Tan B,Badve S,Bigsby RM||Endocrine-related cancer (15:475)||2008|
Estrogen replacement regimen and brain infusion of lipopolysaccharide differentially alter steroid receptor expression in the uterus and hypothalamus.
PA1-310B was used in western blot to study the effects of estrogen replacement and lipopolysaccharide infusion on steroid receptor expression in the uterus and hypothalamus
|Marriott LK,McGann-Gramling KR,Hauss-Wegrzyniak B,Sheldahl LC,Shapiro RA,Dorsa DM,Wenk GL||Endocrine (32:317)||2007|
Brain infusion of lipopolysaccharide increases uterine growth as a function of estrogen replacement regimen: suppression of uterine estrogen receptor-alpha by constant, but not pulsed, estrogen replacement.
PA1-310B was used in western blot to investigate the suppression of uterine estrogen receptor alpha caused by constant or pulsed estrogen replacement.
|Marriott LK,McGann-Gramling KR,Hauss-Wegrzyniak B,Sheldahl LC,Shapiro RA,Dorsa DM,Wenk GL||Endocrinology (148:232)||2007|
Osteoblast differentiation influences androgen and estrogen receptor-alpha and -beta expression.
PA1-310B was used in western blot to investigate the effect of osteoblast differentiation on androgen and estrogen receptor-alpha and -beta expression
|Wiren KM,Chapman Evans A,Zhang XW||The Journal of endocrinology (175:683)||2002|
Cloning of the novel isoform of the estrogen receptor beta cDNA (ERbeta isoform M cDNA) from the human testicular cDNA library.
PA1-310B was used in western blot to identify and characterize a novel estrogen receptor beta cDNA isoform
|Shoda T,Hirata S,Kato J,Hoshi K||The Journal of steroid biochemistry and molecular biology (82:201)||2002|
ERbeta has nongenomic action in caveolae.
PA1-310B was used in western blot to determine whether ERbeta is localized to the endothelial cell plasma membrane
|Chambliss KL,Yuhanna IS,Anderson RG,Mendelsohn ME,Shaul PW||Molecular endocrinology (Baltimore, Md.) (16:938)||2002|
Ductuli efferentes of the male Golden Syrian hamster reproductive tract.
PA1-310B was used in immunohistochemistry to study the morphology and histology of the male Syrian Golden hamster reproductive tract ductuli efferentes
|Ford J,Carnes K,Hess RA||Andrology (2:510)||2014|
Chemoprevention of BBN-Induced Bladder Carcinogenesis by the Selective Estrogen Receptor Modulator Tamoxifen.
PA1-310B was used in immunohistochemistry to study the chemopreventative effects of tamoxifen in a murine model of carcinogen-induced bladder cancer
|George SK,Tovar-Sepulveda V,Shen SS,Jian W,Zhang Y,Hilsenbeck SG,Lerner SP,Smith CL||Translational oncology (6:244)||2013|
Estrogen-dependent regulation of sodium/hydrogen exchanger-3 (NHE3) expression via estrogen receptor β in proximal colon of pregnant mice.
PA1-310B was used in immunohistochemistry and western blot to study estrogen-dependent regulation of sodium/hydrogen exchanger-3 expression in the proximal colon of pregnant mice
|Choijookhuu N,Sato Y,Nishino T,Endo D,Hishikawa Y,Koji T||Histochemistry and cell biology (137:575)||2012|
Sex hormones receptors play a crucial role in the control of femoral and mandibular growth in newborn mice.
PA1-310B was used in immunohistochemistry to investigate the effect of sex hormones receptors on bone development in neonatal mice
|Márquez Hernández RA,Ohtani J,Fujita T,Sunagawa H,Kawata T,Kaku M,Motokawa M,Tanne K||European journal of orthodontics (33:564)||2011|
Distribution of aromatase and sex steroid receptors in the baculum during the rat life cycle: effects of estrogen during the early development of the baculum.
PA1-310B was used in immunohistochemistry to investigate the role of aromatized estrogen in baculum development
|Yonezawa T,Higashi M,Yoshioka K,Mutoh K||Biology of reproduction (85:105)||2011|
Cyclin D1/cdk4, estrogen receptors α and β, in N-methyl-N'-nitro-N-nitrosoguanidine-induced rat gastric carcinogenesis: immunohistochemical study.
PA1-310B was used in immunohistochemistry to investigate the expression of cyclin D1/cdk4 and estrogen receptors in rat gastric carcinogenesis
|Motohashi M,Wakui S,Muto T,Suzuki Y,Shirai M,Takahashi H,Hano H||The Journal of toxicological sciences (36:373)||2011|
Estrogen receptors in the central auditory system of male and female mice.
PA1-310B was used in immunohistochemistry to investigate the distribution of estrogen receptors alpha and beta in murine the central auditory system
|Charitidi K,Canlon B||Neuroscience (165:923)||2010|
Fetal and neonatal exposure to the endocrine disruptor methoxychlor causes epigenetic alterations in adult ovarian genes.
PA1-310B was used in immunohistochemistry to study the effect of early exposure to the endocrine disruptor methoxychlor on epigenetic integrity in adult ovarian genes
|Zama AM,Uzumcu M||Endocrinology (150:4681)||2009|
Blockade of estrogen receptor signaling inhibits growth and migration of medulloblastoma.
PA1-310b was used in immunohistochemistry to study the role of estrogen receptor signaling in medulloblastoma development and migration.
|Belcher SM,Ma X,Le HH||Endocrinology (150:1112)||2009|
Developmental methoxychlor exposure affects multiple reproductive parameters and ovarian folliculogenesis and gene expression in adult rats.
PA1-310B was used in immunohistochemistry to demonstrate the effect of methoxychlor on reproductive development like folliculogenesis
|Armenti AE,Zama AM,Passantino L,Uzumcu M||Toxicology and applied pharmacology (233:286)||2008|
Estrogen receptors alpha and beta are inhibitory modifiers of Apc-dependent tumorigenesis in the proximal colon of Min/+ mice.
PA1-310B was used in immunohistochemistry to investigate the role of estrogen receptor beta during the inhibition of Apc-dependent colon tumorigenesis.
|Cho NL,Javid SH,Carothers AM,Redston M,Bertagnolli MM||Cancer research (67:2366)||2007|
Genetic profiling of aortic allografts: prothymosin alpha as potential target?
PA1-310B was used in immunohistochemistry to identify genes potentially involved in transplant arteriosclerosis following aortic allograft
|Joosten SA,Smit van Dixhoorn MG,Borrias MC,Ham V,Groot Koerkamp MJ,Savolainen-Peltonen HM,Häyry P,Daha MR,Kooten C,Paul LC||Transplant international : official journal of the European Society for Organ Transplantation (18:1010)||2005|
Mechanisms of heat-induced antigen retrieval: analyses in vitro employing SDS-PAGE and immunohistochemistry.
PA1-310B was used in immunohistochemistry to demonstrate the mechanisms of heat-induced antigen retrieval
|Yamashita S,Okada Y||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (53:13)||2005|
The differential ability of the phytoestrogen genistein and of estradiol to induce uterine weight and proliferation in the rat is associated with a substance specific modulation of uterine gene expression.
PA1-310B was used in immunohistochemistry to study the molecular mechanisms underlying the differential induction of rat uterine tissue proliferation by the plant phytoestrogen genistein and by estradiol
|Diel P,Geis RB,Caldarelli A,Schmidt S,Leschowsky UL,Voss A,Vollmer G||Molecular and cellular endocrinology (221:21)||2004|
Effect of estrogen and antiestrogens on the estrogen receptor content in the cochlea of ovariectomized rats.
PA1-310B was used in immunohistochemistry to study the effect of estrogen and antiestrogens on the expression of estrogen receptor alpha and beta in the hearing organs
|Stenberg AE,Simonoska R,Stygar D,Sahlin L,Hultcrantz M||Hearing research (182:19)||2003|
Prostate hyperplasia in a transgenic mouse with prostate-specific expression of prolactin.
PA1-310B was used in immunohistochemistry to investigate the role of prolactin in the prostate hyperplasia.
|Kindblom J,Dillner K,Sahlin L,Robertson F,Ormandy C,Törnell J,Wennbo H||Endocrinology (144:2269)||2003|
Effects of SERM (selective estrogen receptor modulator) treatment on growth and proliferation in the rat uterus.
PA1-310B was used in immunohistochemistry to investigate the effect of SERM treatment on the rat uterus
|Stygar D,Muravitskaya N,Eriksson B,Eriksson H,Sahlin L||Reproductive biology and endocrinology : RB&E (1:null)||2003|
Expression of estrogen receptor-beta protein and mRNA in the cerebellum of the rat.
PA1-310B was used in immunohistochemistry to determine the estrogen receptor beta protein expression in the rat cerebellum
|Price RH,Handa RJ||Neuroscience letters (288:115)||2000|
Localization of estrogen receptor beta-immunoreactivity in astrocytes of the adult rat brain.
PA1-310B was used in immunohistochemistry to investigate the distribution of ER-beta in astrocytes of rat brain
|Azcoitia I,Sierra A,Garcia-Segura LM||Glia (26:260)||1999|
Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.
PA1-310B was used in EMSA to study the prognostic value of estrogen resonse element binding proteins in breast cancer
|Kruer TL,Cummins TD,Powell DW,Wittliff JL||Clinical biochemistry (46:1739)||2013|
Estrogen, predominantly via estrogen receptor α, attenuates postpartum-induced anxiety- and depression-like behaviors in female rats.
PA1-310B was used in immunohistochemistry - frozen section to study the effect of estrogen receptor subtypes to postpartum anxiogenic and depressive responses in rats
|Furuta M,Numakawa T,Chiba S,Ninomiya M,Kajiyama Y,Adachi N,Akema T,Kunugi H||Endocrinology (154:3807)||2013|
Estrogen inhibition of norepinephrine responsiveness is initiated at the plasma membrane of GnRH-producing GT1-7 cells.
PA1-310B was used in immunocytochemistry to investigate whether E2 is able to modify the response of GnRH-producing GT1-7 cells to norepinephrine (NE) stimulation
|Morales A,Gonzalez M,Marin R,Diaz M,Alonso R||The Journal of endocrinology (194:193)||2007|
Estradiol increases proteinuria and angiotensin II type 1 receptor in kidneys of rats receiving L-NAME and angiotensin II.
PA1-310B was used in immunocytochemistry to investigate the effect of E2 on the renovasculature in renal injury
|Oestreicher EM,Guo C,Seely EW,Kikuchi T,Martinez-Vasquez D,Jonasson L,Yao T,Burr D,Mayoral S,Roubsanthisuk W,Ricchiuti V,Adler GK||Kidney international (70:1759)||2006|
Non-nuclear estrogen receptor beta and alpha in the hippocampus of male and female rats.
PA1-310B was used in immunocytochemistry to investigate the expression and subcellular localization of estrogen receptor beta in the rat hippocampus
|Kalita K,Szymczak S,Kaczmarek L||Hippocampus (15:404)||2005|
Expression of functional estrogen receptor beta in locus coeruleus-derived Cath.a cells.
PA1-310B was used in immunocytochemistry to study the expression of estrogen receptor beta in locus coeruleus-derivedcentral-adrenergic-tyrosine-hydroxylase-expressing cells.
|Rincavage HL,McDonnell DP,Kuhn CM||Endocrinology (144:2829)||2003|
Generation and reproductive phenotypes of mice lacking estrogen receptor beta.
PA1-310B was used in immunocytochemistry to study the role of the ERbeta during the ovulation
|Krege JH,Hodgin JB,Couse JF,Enmark E,Warner M,Mahler JF,Sar M,Korach KS,Gustafsson JA,Smithies O||Proceedings of the National Academy of Sciences of the United States of America (95:15677)||1998|
Distribution and localization patterns of estrogen receptor-beta and insulin-like growth factor-1 receptors in neurons and glial cells of the female rat substantia nigra: localization of ERbeta and IGF-1R in substantia nigra.
PA1-310B was used in blocking/activating experiment to investigate the suggested neuroprotective functions of E2 and IGF-1 on SNpc DA neurons from an anaotomical view
|Quesada A,Romeo HE,Micevych P||The Journal of comparative neurology (503:198)||2007|
ER Beta; ER-beta; ESR-Beta; ESR2; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; estrogen receptor beta; estrogen receptor beta 4; nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta
ER-BETA; ER[b]; Erb; Erb2; ERbeta; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2