|Gel Shift (GS)||1-5 µl|
|Immunocytochemistry (ICC)||2-4 µg/ml|
|Immunofluorescence (IF)||0.5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1-2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/mL|
|Western Blot (WB)||1-2 µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 26 publications below|
|Gel Shift (GS)||See 1 publications below|
|ChIP assay (ChIP)||See 2 publications below|
|Immunohistochemistry (IHC)||See 28 publications below|
|Immunocytochemistry (ICC)||See 8 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Non-human primate, Sheep, Rat|
|Published species reactivity||Rat , Pig , Non-human primate , Sheep , Bovine , Hamster , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat ER beta.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-311 detects estrogen receptor (ER) beta from human, non-human primate, mouse, ovine and rat tissues.
PA1-311 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry and gel shift procedures. By Western blot, this antibody detects an ~55-62 kDa protein representing ER beta from rat brain homogenate. Immunohistochemical staining of ER beta in rat cerebellum with PA1-311 results in cytoplasmic staining in Purkinje neurons. PA1-311 efficiently supershifts rat ER beta/ER response element (vitellogenin xenopus A2 gene).
The PA1-311 immunogen is a synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat ER beta.
Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.
The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER Beta; ER-beta; ESR-Beta; ESR2; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta; steroid receptor
Gene Aliases: ER-BETA; ER[b]; ERB; Erb2; ERBETA; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2
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