|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Pig, Non-human primate, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||2-5 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||2-5 µg/mL|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-313 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing ER beta from rat brain homogenate. Immunohistochemical staining of ER beta in rat prostate with PA1-313 results in nuclear staining, whereas nuclear staining may not be observed in human tissue.
The PA1-313 immunogen is a synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta. PA1-313 immunizing peptide (Cat. # PEP-029) is available for use in neutralization and control experiments.
Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.
The Estrogen Receptor beta (ER beta) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. The size and structure of ER beta is very similar to ER alpha, with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as the transactivation domain has diverged significantly. Similar in function to ER alpha, ER beta binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements (ERE's).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Targeting Estrogen Receptor Beta in a Phase 2 Study of High-Dose Estradiol in Metastatic Triple-Negative Breast Cancer: A Wisconsin Oncology Network Study.
PA1-313 was used in immunohistochemistry - paraffin section to analyze a Wisconsin oncology network study of metastatic triple-negative breast cancer where targeting estrogen receptor beta in a phase 2 study of high-dose estradiol
|Wisinski KB,Xu W,Tevaarwerk AJ,Saha S,Kim K,Traynor A,Dietrich L,Hegeman R,Patel D,Blank J,Harter J,Burkard ME||Clinical breast cancer (16:256)||2016|
Prognostic significance of full-length estrogen receptor beta expression in stage I-III triple negative breast cancer.
PA1-313 was used in immunohistochemistry and western blot to examine the association between ERbeta1 and Ki67 expression in non-metastatic triple negative breast cancer
|Shanle EK,Onitilo AA,Huang W,Kim K,Zang C,Engel JM,Xu W,Wisinski KB||American journal of translational research (7:1246)||2015|
Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.
PA1-313 was used in EMSA to study the prognostic value of estrogen resonse element binding proteins in breast cancer
|Kruer TL,Cummins TD,Powell DW,Wittliff JL||Clinical biochemistry (46:1739)||2013|
Estrogen increases survival in an orthotopic model of glioblastoma.
PA1-313 was used in western blot to investigate the effect of estrogen on glioblastoma
|Barone TA,Gorski JW,Greenberg SJ,Plunkett RJ||Journal of neuro-oncology (95:37)||2009|
Differing transcriptional responses to pulsed or continuous estradiol exposure in human umbilical vein endothelial cells.
PA1-313 was used in western blot to study the effect of estradiol on transcriptional responses in human umbilical vein endothelial cells
|Li J,Wang H,Johnson SM,Horner-Glister E,Thompson J,White IN,Al-Azzawi F||The Journal of steroid biochemistry and molecular biology (111:41)||2008|
Changes in transcription profile and cytoskeleton morphology in pelvic ligament fibroblasts in response to stretch: the effects of estradiol and levormeloxifene.
PA1-313 was used in western blot to investigate the effect of mechanical stretch on gene transcription and cytoskeletonal organization in pelvic ligament fibroblasts
|Ewies AA,Elshafie M,Li J,Stanley A,Thompson J,Styles J,White I,Al-Azzawi F||Molecular human reproduction (14:127)||2008|
Single-chain estrogen receptors (ERs) reveal that the ERalpha/beta heterodimer emulates functions of the ERalpha dimer in genomic estrogen signaling pathways.
PA1-313 was used in western blot to study the function of estrogen receptor alpha and beta heterodimer.
|Li X,Huang J,Yi P,Bambara RA,Hilf R,Muyan M||Molecular and cellular biology (24:7681)||2004|
17Beta-estradiol up-regulates vascular endothelial growth factor receptor-2 expression in human myometrial microvascular endothelial cells: role of estrogen receptor-alpha and -beta.
PA1-313 was used in western blot to investigate the role of estrogen receptor-alpha and -beta in human myometrial microvascular endothelial cells.
|Gargett CE,Zaitseva M,Bucak K,Chu S,Fuller PJ,Rogers PA||The Journal of clinical endocrinology and metabolism (87:4341)||2002|
17-Beta-estradiol regulates expression of genes that function in macrophage activation and cholesterol homeostasis.
PA1-313 was used in western blot to investigate the regulation of 17-beta-estradiol on the expression of genes functioning in macrophage activation and cholesterol metabolism
|Kramer PR,Wray S||The Journal of steroid biochemistry and molecular biology (81:203)||2002|
Loss of estrogen receptor beta expression in malignant human prostate cells in primary cultures and in prostate cancer tissues.
PA1-313 was used in western blot to investigate the expression of ER beta and alpha in malignant human prostate cells
|Pasquali D,Rossi V,Esposito D,Abbondanza C,Puca GA,Bellastella A,Sinisi AA||The Journal of clinical endocrinology and metabolism (86:2051)||2001|
Prepubertal physical activity up-regulates estrogen receptor beta, BRCA1 and p53 mRNA expression in the rat mammary gland.
PA1-313 was used in immunohistochemistry to study the effects of physical activity on the expression of the tumor suppressor genes BRCA1, p53 and estrogen receptor-beta in prepupertal rats and the significance for breast cancer
|Wang M,Yu B,Westerlind K,Strange R,Khan G,Patil D,Boeneman K,Hilakivi-Clarke L||Breast cancer research and treatment (115:213)||2009|
Changes in biomarkers of estrogen receptor and growth factor signaling pathways in MCF-7 tumors after short- and long-term treatment with soy and flaxseed.
PA1-313 was used in immunohistochemistry to study the effects of soy and flaxseed on tumor growth and estrogen receptor and growth factor signaling pathways in human breast cancer cells using a murine xenograft model
|Power KA,Chen JM,Saarinen NM,Thompson LU||The Journal of steroid biochemistry and molecular biology (112:13)||2008|
Binding of estrogen receptor beta to estrogen response element in situ is independent of estradiol and impaired by its amino terminus.
PA1-313 was used in immunohistochemistry to study the contribution of distinct amino termini of estrogen receptors towards their DNA response element binding and subsequent transcription efficacies.
|Huang J,Li X,Maguire CA,Hilf R,Bambara RA,Muyan M||Molecular endocrinology (Baltimore, Md.) (19:2696)||2005|
Nuclear estrogen receptor beta in lung cancer: expression and survival differences by sex.
PA1-313 was used in immunohistochemistry to demonstrate the sex-related expression of nuclear estrogen receptor beta in lung cancer.
|Schwartz AG,Prysak GM,Murphy V,Lonardo F,Pass H,Schwartz J,Brooks S||Clinical cancer research : an official journal of the American Association for Cancer Research (11:7280)||2005|
Estrogen receptors in the uterus and ovarian follicles of gilts treated with dihydrotestosterone.
PA1-313 was used in immunohistochemistry to investigate the expression of estrogen receptor alpha and beta in the uterus and ovarian follicles
|Cárdenas H,Pope WF||Domestic animal endocrinology (29:523)||2005|
Oestrogen receptor beta: what it means for patients with breast cancer.
PA1-313 was used in immunohistochemistry to review the significance of oestrogen receptor-beta in breast cancer patients
|Speirs V,Carder PJ,Lane S,Dodwell D,Lansdown MR,Hanby AM||The Lancet. Oncology (5:174)||2004|
Estrogen regulates adrenal angiotensin AT1 receptors by modulating AT1 receptor translation.
PA1-313 was used in immunohistochemistry to investigate the molecular mechanisms for estrgen regulation of AT1R gene expression in the rat adrenal gland.
|Wu Z,Maric C,Roesch DM,Zheng W,Verbalis JG,Sandberg K||Endocrinology (144:3251)||2003|
Estrogen receptors in the normal adult and developing human inner ear and in Turner's syndrome.
PA1-313 was used in immunohistochemistry to investigate the expression and activity of estrogen receptors in human sensory tissues
|Stenberg AE,Wang H,Fish J,Schrott-Fischer A,Sahlin L,Hultcrantz M||Hearing research (157:87)||2001|
Different regulation of oestrogen receptors alpha and beta in the human cervix at term pregnancy.
PA1-313 was used in immunohistochemistry to study the expression change of the two ERs in the cervix from women at term pregnancy and immediately post-partum and to try to find its relationship with cervical ripening
|Wang H,Stjernholm Y,Ekman G,Eriksson H,Sahlin L||Molecular human reproduction (7:293)||2001|
Estrogen receptor beta is coexpressed with ERalpha and PR and associated with nodal status, grade, and proliferation rate in breast cancer.
PA1-313 was used in immunohistochemistry the study the expression of estrogen receptor beta in normal and pathological human tissues.
|Järvinen TA,Pelto-Huikko M,Holli K,Isola J||The American journal of pathology (156:29)||2000|
|Non-human primate||2 ug/ml||
Identification and developmental expression of the estrogen receptor alpha and beta in the baboon fetal adrenal gland.
PA1-313 was used in immunohistochemistry to study the expression of the estrogen receptor alpha and beta in the adrenal gland
|Albrecht ED,Babischkin JS,Davies WA,Leavitt MG,Pepe GJ||Endocrinology (140:5953)||1999|