PA1-313 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing ER beta from rat brain homogenate. Immunohistochemical staining of ER beta in rat prostate with PA1-313 results in nuclear staining, whereas nuclear staining may not be observed in human tissue.
The PA1-313 immunogen is a synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta. PA1-313 immunizing peptide (Cat. # PEP-029) is available for use in neutralization and control experiments.
Recombinant ER beta expressed in baculovirus system (Cat. # RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.
Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER beta; ER-beta; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta
Gene Aliases: ER-BETA; ER[b]; Erb; Erb2; ERbeta; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2