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Exonuclease 1 Polyclonal Antibody

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Exonuclease 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Exonuclease 1 Polyclonal Antibody, Unconjugated (bs-13119R) at 1:400 overnight at 4°C, DAB staining.

Exonuclease 1 Antibody in Western Blot (WB) — Lane 1: Mouse testis lysates; Lane 2: Mouse thymus lysates probed with Exonuclease 1 Polyclonal Antibody, Unconjugated (bs-13119R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.

Product Details

Applications

Tested Dilution

Publications

Immunofluorescence (IF)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Western Blot (WB)

1:300

Product Specifications

Species

Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Exonuclease 1.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 50% glycerol, 1% BSA

Contains

0.09% sodium azide

Storage conditions

-20°C

Target Information

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Required for muscle atrophy following denervation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Exonuclease 1; Exonuclease I; hExo1; hExoI; mExo1; rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1

Gene Aliases: 5730442G03Rik; EXO1; EXOI; HEX1; hExoI; Msa

UniProt ID: (Human) Q9UQ84, (Mouse) Q9QZ11

Entrez Gene ID: (Human) 9156, (Rat) 305000, (Mouse) 26909

Molecular Function: DNA binding protein damaged DNA-binding protein endodeoxyribonuclease exodeoxyribonuclease hydrolase nuclease nucleic acid binding

Function(s)

DNA synthesis involved in DNA repair strand displacement humoral immune response mediated by circulating immunoglobulin DNA replication DNA repair mismatch repair DNA recombination somatic hypermutation of immunoglobulin genes isotype switching meiotic cell cycle RNA phosphodiester bond hydrolysis, endonucleolytic regulation of signal transduction by p53 class mediator DNA catabolic process, endonucleolytic DNA catabolic process, exonucleolytic biological_process immune system process nucleobase-containing compound metabolic process cellular response to DNA damage stimulus nucleic acid phosphodiester bond hydrolysis

Process(es)

DNA binding chromatin binding RNA-DNA hybrid ribonuclease activity exonuclease activity protein binding 5'-3' exonuclease activity 5'-3' exodeoxyribonuclease activity single-stranded DNA 5'-3' exodeoxyribonuclease activity metal ion binding flap endonuclease activity double-stranded DNA 5'-3' exodeoxyribonuclease activity molecular_function structure-specific DNA binding catalytic activity nuclease activity endonuclease activity hydrolase activity hydrolase activity, acting on ester bonds phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity

Exonuclease 1 Polyclonal Antibody

Product Details

Target Information

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Bioinformatics

Material safety data sheets (MSDS)

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