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Invitrogen
F4/80 Polyclonal Antibody
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FIGURE: 1 / 8
F4/80 Antibody (PA5-32399) in ICC/IF
Biotechnology and bioengineering 2020 -
1 Figure Transplantation of adipose tissue with hIL-4 PLG implants. (a) Transplantation of implants into eWAT fat pads; left-implants placed in each eWAT fat pad before folding fat pad over the implant and returning to abdominal cavity: right--implants in eWAT fat pads 2 weeks after transplantation. (b) Study design timeline. (c) Expression of hIL-4 in murine adipose tissue measured by qRT-PCR at 2 and 10 w... View More
Product Details
PA5-32399
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from N-terminus of mouse F4/80 protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is human lung or mouse liver tissue.
Target Information
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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