Invitrogen

FAAH Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Protocols

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FAAH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human breast cancer, using FAAH (Product # PA5-32183) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

FAAH Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of LNCaP (Lane 1), PC-3 (Lane 2), DU 145 (Lane 3), MCF7 (Lane 4), A-431 (Lane 5), HEK 293 (Lane 6), tissue extracts of Mouse Liver (Lane 7) and Rat Liver (Lane 8). The blot was probed with Anti- FAAH Polyclonal Antibody (Product # PA5-32183, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 60 kDa band corresponding to FAAH was detected across cell lines tested except PC-3 and DU 145 which are reported to be negative for FAAH expression in comparison to LNCaP cell line.

FAAH Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of FAAH was observed in LNCaP, PC-3 and DU 145 where PC-3 and DU 145 are reported to be negative for FAAH expression using Anti-FAAH Monoclonal Antibody (Product # PA5-32183) in Western Blot. {RE}

Product Details

PA5-32183

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 467 and 579 of Human FAAH View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 1% BSA, 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2549656

Product Specific Information

Recommended positive controls: MCF-7.

Predicted reactivity: Mouse (84%), Rat (82%), Pig (85%), Bovine (80%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Degrades bioactive fatty acid amides like oleamide, the endogenous levels of cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: amide hydrolase; Anandamide amidase; Anandamide amidohydrolase 1; FA2H; fatty acid amide hydrolase protein; Fatty acid ester hydrolase; Fatty-acid amide hydrolase 1; MGC102823; MGC138146; Oleamide hydrolase; Oleamide hydrolase 1

Gene Aliases: AW412498; FAAH; FAAH-1; FAAH1; PSAB

UniProt ID: (Human) O00519, (Rat) P97612, (Mouse) O08914

Entrez Gene ID: (Human) 2166, (Rat) 29347, (Mouse) 14073

Molecular Function: hydrolase