|Tested species reactivity||Mouse, Non-human primate, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human FANCD2 fusion protein (N-terminal fragment).|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||2.5-5.0 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2.5-5 µg/ml|
|Western Blot (WB)||1:10,000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form).
Additional bands may be seen at lower molecular weights.
In immunofluorescence, this has been tested in human MMC and IR-treated MEF cells.
Fanconi anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANCD2, targeting nuclear foci containing the BRCA1 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.