Invitrogen

FAP Monoclonal Antibody (F11-24), eBioscience™

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Published Figures (2)

FIGURE: 1 / 2

FAP Antibody (BMS168) in Flow

Cancer science 2023 - 2 FIGURE Verification of fibroblast activation protein alpha (FAP-alpha) overexpression, and human and murine FAP-alpha cross-reactivity of AF3715. (A) Immunoblot analysis confirming FAP-alpha overexpression in 231-FAP and HT-1080-FAP cells. (B) Relative FAP-alpha mRNA expression levels (mean +- SD) of prostate cancer-associated fibroblasts (PCAFs), pancreatic cancer-associated fibroblasts (CAF35), MDA-MB-2... View More

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FAP Antibody in Immunohistochemistry (IHC) — Published figure using FAP monoclonal antibody (Product # BMS168) in Immunohistochemistry

Product Details

BMS168

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

Assay-Dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

F11-24

Immunogen

Rec. Human FAP

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

RRID

AB_10597443

Product Specific Information

Description: BMS168 (F11-24) specifically reacts with Fibroblast Activation Protein (FAP), a cell surface glycoprotein of reactive human fibroblast. Among the normal adult organs tested BMS168 does not show immunoreactivity in immunohistological studies with either resting fibroblasts (fibrocytes) of the normal organ mesenchyme or the parenchymal cells. The single exception noted is a subset of endocrine cells in the pancreatic islets, presumably glucagon-producing A cells.

Applications Tested: Flow Cytometry, Immunohistochemistry, Immunoprecipitation.

Target Information

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluating near-infrared photoimmunotherapy for targeting fibroblast activation protein-α expressing cells in vitro and in vivo.

Cancer science

Jin J,Barnett JD,Krishnamachary B,Mironchik Y,Luo CK,Kobayashi H,Bhujwalla ZM

Published figure using FAP monoclonal antibody (Product # BMS168) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2023

N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer.

International journal of oncology

Pan S,Deng Y,Fu J,Zhang Y,Zhang Z,Qin X

BMS168 was used in Immunocytochemistry to investigate the role of exosomal miRNAs in 5‚Fluorouracil sensitivity and resistance in colorectal cancer.

Tue Feb 01 00:00:00 EST 2022

TNF antagonist sensitizes synovial fibroblasts to ferroptotic cell death in collagen-induced arthritis mouse models.

Nature communications

Wu J,Feng Z,Chen L,Li Y,Bian H,Geng J,Zheng ZH,Fu X,Pei Z,Qin Y,Yang L,Zhao Y,Wang K,Chen R,He Q,Nan G,Jiang X,Chen ZN,Zhu P

BMS168 was used in Immunohistochemistry, Flow Cytometry to imply that the combination of TNF inhibitors and ferroptosis inducers may serve as a potential candidate for RA therapy.

Thu Feb 03 00:00:00 EST 2022

TNF antagonist sensitizes synovial fibroblasts to ferroptotic cell death in collagen-induced arthritis mouse models.

Nature communications

Wu J,Feng Z,Chen L,Li Y,Bian H,Geng J,Zheng ZH,Fu X,Pei Z,Qin Y,Yang L,Zhao Y,Wang K,Chen R,He Q,Nan G,Jiang X,Chen ZN,Zhu P

BMS168 was used in Immunohistochemistry, Flow Cytometry to imply that the combination of TNF inhibitors and ferroptosis inducers may serve as a potential candidate for RA therapy.

Thu Feb 03 00:00:00 EST 2022

Endogenous Galectin-9 Suppresses Apoptosis in Human Rheumatoid Arthritis Synovial Fibroblasts.

Scientific reports

Pearson MJ,Bik MA,Ospelt C,Naylor AJ,Wehmeyer C,Jones SW,Buckley CD,Gay S,Filer A,Lord JM

BMS168 was used in Immunohistochemistry-immunofluorescence to determine the role of endogenous Galectins in the inflammatory pathology of the rheumatoid arthritis synovium in humans.

Mon Aug 27 00:00:00 EDT 2018

IL-6/STAT3 axis initiated CAFs via up-regulating TIMP-1 which was attenuated by acetylation of STAT3 induced by PCAF in HCC microenvironment.

Cellular signalling

Zheng X,Xu M,Yao B,Wang C,Jia Y,Liu Q

BMS168 was used in western blot to examine the mechanisms by which IL-6/STAT3 signaling and TIMP-1 modulate the crosstalk between tumor cells and cancer associated fibroblasts in the tumor microenvironment

Thu Sep 01 00:00:00 EDT 2016

COX/mPGES-1/PGE2 pathway depicts an inflammatory-dependent high-risk neuroblastoma subset.

Proceedings of the National Academy of Sciences of the United States of America

Larsson K,Kock A,Idborg H,Arsenian Henriksson M,Martinsson T,Johnsen JI,Korotkova M,Kogner P,Jakobsson PJ

BMS168 was used in immunohistochemistry to study the involvement of COX/mPGES-1/PGE2 pathway in tumor inflammatory environment

Tue Jun 30 00:00:00 EDT 2015

Hierarchical paracrine interaction of breast cancer associated fibroblasts with cancer cells via hMAPK-microRNAs to drive ER-negative breast cancer phenotype.

Cancer biology & therapy

Shah SH,Miller P,Garcia-Contreras M,Ao Z,Machlin L,Issa E,El-Ashry D

BMS168 was used in immunocytochemistry to study the role of MAPK-microRNA in the interaction between cancer cells and neighboring fibroblasts

Mon Sep 12 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 170 kDa melanoma membrane-bound gelatinase; Antiplasmin-cleaving enzyme FAP, soluble form; APCE; Dipeptidyl peptidase FAP; FAPalpha; Fibroblast activation protein alpha; fibroblast activation protein, alpha; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Prolyl endopeptidase FAP; Seprase; Serine integral membrane protease; SIMP; Surface-expressed protease

Gene Aliases: DPPIV; FAP; FAPA; SIMP

UniProt ID: (Human) Q12884

Entrez Gene ID: (Human) 2191

Molecular Function: serine protease

Function(s)

angiogenesis proteolysis cell adhesion regulation of collagen catabolic process negative regulation of extracellular matrix disassembly endothelial cell migration proteolysis involved in cellular protein catabolic process regulation of fibrinolysis negative regulation of cell proliferation involved in contact inhibition positive regulation of cell cycle arrest mitotic cell cycle arrest melanocyte proliferation positive regulation of execution phase of apoptosis melanocyte apoptotic process negative regulation of extracellular matrix organization

Process(es)

protease binding endopeptidase activity metalloendopeptidase activity serine-type endopeptidase activity integrin binding protein binding peptidase activity serine-type peptidase activity dipeptidyl-peptidase activity protein homodimerization activity protein dimerization activity

FAP Monoclonal Antibody (F11-24), eBioscience™

FAP Antibody (BMS168) in Flow

Product Details

BMS168

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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