Invitrogen

FAS Monoclonal Antibody (5F7)

Product Image Gallery

FAS Antibody — Fig. 4 OPG binds to Fas, which is increased in IPAH lung and right ventricle. Panel ( a ) demonstrates confirmed protein binding between OPG and syndecan-1 (SDC-1), RANKL (TNFSF11), Growth Associated Protein 43 (GAP43), Fas, IL1-receptor accessory protein (IL-1RAcP) and transmembrane protease, serine 11D. b TaqMan expression of Fas, IL-1RAcP and GAP43 in control (white bars, 0.2% FCS) and OPG-stimulated (blue bars, 50 ng ml -1 ) purchased PASMCs, and ( c ) PASMCs from patients with IPAH (grey bars) and healthy controls (white bars). d Anti-Fas co-immunoprecipitation of OPG in endogenous primary human PASMC lysates or recombinant protein replicated 3 times. e OPG and Fas are expressed within remodelled pulmonary arteries and the right ventricle of patients with IPAH. TaqMan expression of Fas in whole lung RNA (f) and protein expression in lung sections ( g ) isolated from control (saline), monocrotaline (d28), control (normoxia) and SuHx (wk9) rats. TaqMan expression data normalised using DeltaDeltaCT with 18 s rRNA as the endogenous control gene. Bars represent the mean with error bars showing the standard error of the mean. Panel ( c ) n = 4 and panel ( d ) n = 3 from three individual donors, dots represent experimental repeats. * p < 0.05, ** p < 0.01, *** p < 0.001 following one-way ANOVA with Bonferroni's multiple comparisons post hoc test. When there were only two groups, unpaired t-tests were used. Scale bar represents 25 um

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 1 publication 1 publication

ELISA (ELISA)

1:1000

Immunoprecipitation (IP)

1:1000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5F7

Immunogen

Purified recombinant FAS protein.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.01% sodium azide

Storage conditions

4°C short term, -80°C long term

RRID

AB_1073510

Product Specific Information

MA1-7622 detects FAS from human, rat and mouse samples.

MA1-7622 has been successfully used in ELISA, Western blot and immunoprecipitation procedures.

The MA1-7622 immunogen is a purified recombinant FAS protein.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily member); Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas antigen (ATP1); Fas receptor; FASLG receptor; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; Tumor necrosis factor receptor superfamily, member 6

Gene Aliases: AI196731; ALPS1A; APO-1; APO1; APT1; CD95; FAS; FAS1; FASTM; lpr; TNFR6; TNFRSF6

UniProt ID: (Human) P25445, (Rat) Q63199, (Mouse) P25446

Entrez Gene ID: (Human) 355, (Rat) 246097, (Mouse) 14102

Molecular Function: cytokine receptor receptor tumor necrosis factor receptor