Invitrogen

FAS Monoclonal Antibody (5F7)

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FAS Antibody in Western Blot (WB) — Figure 1 Upregulation of miR-15a/b and miR-16 by hyperosmolarity in perfused rat liver. Rat livers were perfused with normoosmotic medium (305 mosmol/l), hyperosmotic medium (385 mosmol/l) or hypoosmotic medium (225 mosmol) for up to 180 min. Samples were taken at the time points indicated. (a) miR-15a is significantly upregulated after 60 and 120 minutes of hyperosmotic conditions, whereas a stable expression is observed under hypo- and normoosmotic (305 mosmol/l) conditions (* p < 0.05; **p < 0.01; ***p < 0.001). (b) miR-15b is significantly upregulated at 60 minutes of hyperosmotic perfusion, while it is stably expressed under normoosmotic and hypoosmotic conditions. (c) miR-16 is significantly upregulated under hyperosmolarity. Statistical analysis was carried out by unpaired student's t-test. Data are shown as average +- S.E.M. of 5 independent experiments. The values of unstimulated controls (T0) were set arbitrarily to 100 . (d) CD95 was immunoprecipitated and activating CD95-tyrosine phosphorylation (P-CD95-Y) and caspase 3 cleavage were analysed by Western blot using specific antibodies. Total CD95 and gamma-tubulin served as respective loading controls. Representative immunoblots from 3 independent experiments are depicted. Hyperosmolarity-induced activation of the CD95 was already detected after 30 min, whereas cleavage of caspase 3 occurred 120 min after exposure to hyperosmolarity. Normoosmotic- and hypoosmotic perfusion of rat liver do not lead to initiatio

FAS Antibody in Immunoprecipitation (IP) — Fig. 4 OPG binds to Fas, which is increased in IPAH lung and right ventricle. Panel ( a ) demonstrates confirmed protein binding between OPG and syndecan-1 (SDC-1), RANKL (TNFSF11), Growth Associated Protein 43 (GAP43), Fas, IL1-receptor accessory protein (IL-1RAcP) and transmembrane protease, serine 11D. b TaqMan expression of Fas, IL-1RAcP and GAP43 in control (white bars, 0.2% FCS) and OPG-stimulated (blue bars, 50 ng ml -1 ) purchased PASMCs, and ( c ) PASMCs from patients with IPAH (grey bars) and healthy controls (white bars). d Anti-Fas co-immunoprecipitation of OPG in endogenous primary human PASMC lysates or recombinant protein replicated 3 times. e OPG and Fas are expressed within remodelled pulmonary arteries and the right ventricle of patients with IPAH. TaqMan expression of Fas in whole lung RNA (f) and protein expression in lung sections ( g ) isolated from control (saline), monocrotaline (d28), control (normoxia) and SuHx (wk9) rats. TaqMan expression data normalised using DeltaDeltaCT with 18 s rRNA as the endogenous control gene. Bars represent the mean with error bars showing the standard error of the mean. Panel ( c ) n = 4 and panel ( d ) n = 3 from three individual donors, dots represent experimental repeats. * p < 0.05, ** p < 0.01, *** p < 0.001 following one-way ANOVA with Bonferroni's multiple comparisons post hoc test. When there were only two groups, unpaired t-tests were used. Scale bar represents 25 um

Product Details

MA1-7622

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
ELISA (ELISA)	1:1,000	-
Immunoprecipitation (IP)	2-5 µg/10^6 cells	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Not Applicable
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	5F7
Immunogen	Purified recombinant FAS protein.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.2
Contains	0.01% sodium azide
Storage conditions	4°C short term, -80°C long term
RRID	AB_1073510

Product Specific Information

MA1-7622 detects FAS from human, rat and mouse samples.

MA1-7622 has been successfully used in ELISA, Western blot and immunoprecipitation procedures.

The MA1-7622 immunogen is a purified recombinant FAS protein.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension.

Nature communications

Arnold ND,Pickworth JA,West LE,Dawson S,Carvalho JA,Casbolt H,Braithwaite AT,Iremonger J,Renshall L,Germaschewski V,McCourt M,Bland-Ward P,Kowash H,Hameed AG,Rothman AMK,Frid MG,Roger Thompson AA,Evans HR,Southwood M,Morrell NW,Crossman DC,Whyte MKB,Stenmark KR,Newman CM,Kiely DG,Francis SE,Lawrie A

Published figure using FAS monoclonal antibody (Product # MA1-7622) in Immunoprecipitation

Fri Nov 15 00:00:00 EST 2019

Hyperosmotic stress activates the expression of members of the miR-15/107 family and induces downregulation of anti-apoptotic genes in rat liver.

Scientific reports

Santosa D,Castoldi M,Paluschinski M,Sommerfeld A,Häussinger D

Published figure using FAS monoclonal antibody (Product # MA1-7622) in Western Blot

Tue Jul 21 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily member); Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; FASLG receptor; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

Gene Aliases: AI196731; ALPS1A; APO-1; APO1; APT1; CD95; FAS; FAS1; FASTM; lpr; TNFR6; TNFRSF6

UniProt ID: (Human) P25445, (Mouse) P25446

Entrez Gene ID: (Human) 355, (Mouse) 14102

Molecular Function: transmembrane signal receptor