Bethyl Laboratories

FASN Polyclonal Antibody

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FASN Antibody in Immunohistochemistry (IHC) — **Detection of human FASN by immunohistochemistry.** *Sample:* FFPE section of human prostate carcinoma. *Antibody:* Affinity purified rabbit anti-FASN (Cat. No. A301-323A) used at a dilution of 1:200 (1µg/ml). *Detection:* DAB.

FASN Antibody in Western Blot (WB) — **Detection of human FASN by western blot and immunoprecipitation.** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-FASN antibody A301-323A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate. FASN was also immunoprecipitated by rabbit anti-FAS antibodyA301-324A, which recognizes a downstream epitope. *Detection:* Chemiluminescence with exposure times of 30 seconds (A and B).

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:100-1:500

Immunoprecipitation (IP)

2-5 µg/mg lysate

Western Blot (WB)

1:2,000-1:10,000

Product Specifications

Species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 1175 and 1225

Conjugate

Unconjugated

Form

liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; MGC14367; MGC15706; Oleoyl-[acyl-carrier-protein] hydrolase; short chain dehydrogenase/reductase family 27X, member 1

Gene Aliases: FAS; FASN; OA-519; SDR27X1

UniProt ID: (Human) P49327

Entrez Gene ID: (Human) 2194

Molecular Function: acyltransferase dehydrogenase esterase hydrolase ligase methyltransferase oxidoreductase transferase