Invitrogen

FASN Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (27) FASN antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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FASN Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of FASN in MCF-7 cells using FASN Polyclonal Antibody (Product # PA1-32355) (Green) at a dilution of 1:2000. Red : Tubulin. Blue: DAPI.

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of FASN in 3-week-old chicken hypothalmus tissue using FASN Polyclonal Antibody (Product # PA1-32355).

FASN Antibody in Western Blot (WB) — Western blot was performed using Anti-FASN Polyclonal Antibody (Product # PA1-32355) and a ~260kDa band corresponding to FASN was observed across cell lines tested . Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A549 (Lane 2), HeLa (Lane 3), Caco-2 (Lane 4), NTERA-2 cl.D1 (Lane 5), PC-3 (Lane 6) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

FASN Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with FASN siRNA and decrease in signal intensity was observed in Western Blot application using Anti-FASN Polyclonal Antibody (Product # PA1-32355). {KD}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:2000

Immunofluorescence (IF)

1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunoprecipitation (IP)

1:100

Western Blot (WB)

1:1000

Product Specifications

Species

Chicken, Hamster, Human, Mouse, Non-human primate, Pig, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An internal synthetic peptide, conjugated to KLH, made near the N-terminus of the mouse FAS protein sequence.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2231412

Product Specific Information

PA1-32355 detects Fatty Acid Synthase from mouse, porcine, human, monkey and rat samples.

Target Information

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; fatty acid synthase (EC 3.2.1.23); MGC14367; MGC15706; Oleoyl-[acyl-carrier-protein] hydrolase; short chain dehydrogenase/reductase family 27X, member 1; thioesterase

Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1

UniProt ID: (Human) P49327, (Mouse) P19096, (Rat) P12785

Entrez Gene ID: (Human) 2194, (Pig) 397561, (Chicken) 396061, (Chinese hamster) 100689207, (Mouse) 14104, (Rat) 50671

Molecular Function: acyltransferase dehydrogenase esterase hydrolase ligase methyltransferase oxidoreductase transferase