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Invitrogen
FASN Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
FASN Antibody (PA1-32355) in WB
Western blot was performed using Anti-FASN Polyclonal Antibody (Product # PA1-32355) and a ~260kDa band corresponding to FASN was observed across cell lines tested . Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A549 (Lane 2), HeLa (Lane 3), Caco-2 (Lane 4), NTERA-2 cl.D1 (Lane 5), PC-3 (Lane 6) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot... View More
Product Details
PA1-32355
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
An internal synthetic peptide, conjugated to KLH, made near the N-terminus of the mouse FAS protein sequence.
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2231412
Product Specific Information
PA1-32355 detects Fatty Acid Synthase from mouse, porcine, human, monkey and rat samples.
Target Information
Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; Acyl-[acyl-carrier-protein] hydrolase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; fatty acid synthase (EC 3.2.1.23); MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; thioesterase; Type I FAS; Type I Fatty Acid Synthase
Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1
UniProt ID: (Human) P49327, (Mouse) P19096, (Rat) P12785
Entrez Gene ID: (Human) 2194, (Pig) 397561, (Chicken) 396061, (Chinese hamster) 100689207, (Mouse) 14104, (Rat) 50671
osteoblast differentiation
acetyl-CoA metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
pantothenate metabolic process
mammary gland development
positive regulation of cellular metabolic process
long-chain fatty-acyl-CoA biosynthetic process
oxidation-reduction process
cellular response to interleukin-4
cell-cell adhesion
lipid metabolic process
metabolic process
biosynthetic process
lactate metabolic process
positive regulation of appetite
lipid biosynthetic process
fatty acid synthase activity
[acyl-carrier-protein] S-acetyltransferase activity
[acyl-carrier-protein] S-malonyltransferase activity
3-oxoacyl-[acyl-carrier-protein] synthase activity
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity
oleoyl-[acyl-carrier-protein] hydrolase activity
protein binding
drug binding
myristoyl-[acyl-carrier-protein] hydrolase activity
palmitoyl-[acyl-carrier-protein] hydrolase activity
phosphopantetheine binding
protein homodimerization activity
poly(A) RNA binding
enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity
NADPH binding
cadherin binding involved in cell-cell adhesion
3-oxo-pimeloyl-[acp] methyl ester reductase activity
single-stranded DNA binding
(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity
3-oxo-glutaryl-[acp] methyl ester reductase activity
catalytic activity
zinc ion binding
acyl-[acyl-carrier-protein] hydrolase activity
oxidoreductase activity
transferase activity
hydrolase activity
hydrolase activity, acting on ester bonds
lyase activity
3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
identical protein binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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