Invitrogen

FASN Polyclonal Antibody

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FASN Antibody in Western Blot (WB) — Western blot analysis of Mouse Brain Tissue Lysate using Product # PA5-19509, Fatty Acid Synthase primary antibody at a dilution of 1 µg/mL. Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

FASN Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Figure 5 Neonatal hyperoxia represses fatty acid synthesis enzymes in murine atrial cardiomyocytes. ( A , B , D , and E ). Sections of left atrial appendages from P4 neonates exposed to room air ( A and B ) or hyperoxia ( D and E ) costained for FASN (red, A and B ) or SCD1 (red, D and E ) and TNNT2 (green, A , B , D , and E ). Sections were also stained with DAPI to label nuclei (blue, A , B , D , and E ). Arrows and arrowheads show TNNT2 + cardiomyocytes in left atrial appendage and LV, respectively. Scale bars: 200 mum. ( C and F ) Graphs show relative staining intensities for Fasn ( C ) and Scd1 ( F ) measured using NIH ImageJ 2.0/Fiji. ( C and F ) Room air, n = 5; hyperoxia, n = 5. Box plots show median values and inner quartiles. Whiskers show the range of values. Circles show values for individual room air- and hyperoxia-exposed mice, respectively. F tests were used to determine if samples had equal or unequal variances. P values are the results of unpaired 2-tailed t tests.

Product Details

PA5-19509

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-Dependent	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	Assay-Dependent	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	1 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 2450 to the C-terminus of Mouse Fatty Acid Synthase. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.9 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10985006

Product Specific Information

This antibody is predicted to react with chicken and cow based on sequence homology.

Target Information

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neonatal hyperoxia inhibits proliferation and survival of atrial cardiomyocytes by suppressing fatty acid synthesis.

JCI insight

Cohen ED,Yee M,Porter GA,Ritzer E,McDavid AN,Brookes PS,Pryhuber GS,O'Reilly MA

PA5-19509 was used in Immunohistochemistry (PFA fixed) to show that hyperoxia also reduces cardiomyocyte proliferation and survival in the left atrium and causes diastolic heart failure by disrupting its filling of the left ventricle.

Mon Mar 08 00:00:00 EST 2021

Neonatal hyperoxia inhibits proliferation and survival of atrial cardiomyocytes by suppressing fatty acid synthesis.

JCI insight

Cohen ED,Yee M,Porter GA,Ritzer E,McDavid AN,Brookes PS,Pryhuber GS,O'Reilly MA

Mon Mar 08 00:00:00 EST 2021

Bioinformatics

Protein Aliases: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; Acyl-[acyl-carrier-protein] hydrolase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; Type I FAS; Type I Fatty Acid Synthase

Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1

UniProt ID: (Human) P49327, (Mouse) P19096, (Rat) P12785

Entrez Gene ID: (Human) 2194, (Mouse) 14104, (Rat) 50671

Function(s)