Invitrogen

FASN Polyclonal Antibody

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Antibody Testing Data (1)
Published Figures (1)

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FASN Antibody (PA5-19509) in WB

Western blot analysis of Mouse Brain Tissue Lysate using Product # PA5-19509, Fatty Acid Synthase primary antibody at a dilution of 1 µg/mL. Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Product Image Gallery

FASN Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Figure 5 Neonatal hyperoxia represses fatty acid synthesis enzymes in murine atrial cardiomyocytes. ( A , B , D , and E ). Sections of left atrial appendages from P4 neonates exposed to room air ( A and B ) or hyperoxia ( D and E ) costained for FASN (red, A and B ) or SCD1 (red, D and E ) and TNNT2 (green, A , B , D , and E ). Sections were also stained with DAPI to label nuclei (blue, A , B , D , and E ). Arrows and arrowheads show TNNT2 + cardiomyocytes in left atrial appendage and LV, respectively. Scale bars: 200 mum. ( C and F ) Graphs show relative staining intensities for Fasn ( C ) and Scd1 ( F ) measured using NIH ImageJ 2.0/Fiji. ( C and F ) Room air, n = 5; hyperoxia, n = 5. Box plots show median values and inner quartiles. Whiskers show the range of values. Circles show values for individual room air- and hyperoxia-exposed mice, respectively. F tests were used to determine if samples had equal or unequal variances. P values are the results of unpaired 2-tailed t tests.

Product Details

PA5-19509

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 2450 to the C-terminus of Mouse Fatty Acid Synthase.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.9 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_10985006

Product Specific Information

This antibody is predicted to react with chicken and cow based on sequence homology.

Target Information

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neonatal hyperoxia inhibits proliferation and survival of atrial cardiomyocytes by suppressing fatty acid synthesis.

JCI insight

Cohen ED,Yee M,Porter GA,Ritzer E,McDavid AN,Brookes PS,Pryhuber GS,O'Reilly MA

Published figure using FASN polyclonal antibody (Product # PA5-19509) in Immunohistochemistry (PFA fixed)

Mon Mar 08 00:00:00 EST 2021

Neonatal hyperoxia inhibits proliferation and survival of atrial cardiomyocytes by suppressing fatty acid synthesis.

JCI insight

Cohen ED,Yee M,Porter GA,Ritzer E,McDavid AN,Brookes PS,Pryhuber GS,O'Reilly MA

Published figure using FASN polyclonal antibody (Product # PA5-19509) in Immunohistochemistry (PFA fixed)

Mon Mar 08 00:00:00 EST 2021

Bioinformatics

Protein Aliases: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; Acyl-[acyl-carrier-protein] hydrolase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; Type I FAS; Type I Fatty Acid Synthase

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Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1

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UniProt ID: (Human) P49327, (Mouse) P19096, (Rat) P12785

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Entrez Gene ID: (Human) 2194, (Mouse) 14104, (Rat) 50671

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Function(s)

osteoblast differentiation acetyl-CoA metabolic process fatty acid metabolic process fatty acid biosynthetic process pantothenate metabolic process mammary gland development positive regulation of cellular metabolic process long-chain fatty-acyl-CoA biosynthetic process oxidation-reduction process cellular response to interleukin-4 cell-cell adhesion lipid metabolic process metabolic process biosynthetic process lipid biosynthetic process

Process(es)

Performance Guarantee

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FASN Polyclonal Antibody

FASN Antibody (PA5-19509) in WB

Product Details

PA5-19509

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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