Invitrogen

FASN Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (34) FASN antibodies

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Protocols

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FASN Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of FASN was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: FASN Polyclonal Antibody (Product # PA5-22061) diluted at 1:500. Blue: Hoechst 33342 staining.

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — FASN Polyclonal Antibody detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Fatty Acid Synthase stained by FASN Polyclonal Antibody (Product # PA5-22061) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

FASN Antibody in Western Blot (WB) — CRISPR-Cas9 mediated genome editing ofFASN (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR806223_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of FASN was performed by loading 30 µg of HepG2 wild type (Lane 1), HepG2 Cas9 (Lane 2) and HepG2 Cas9 cells transduced with FASN Lentiviral sgRNA (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-FASN Polyclonal Antibody (Product # PA5-22061) using 1:1000 dilution and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177 1:5000 dilution).Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). A loss of signal in sgRNA transduced cells using the LentiArray™ CRISPR product line confirms that antibody is specific toFASN (Fig (b)).

FASN Antibody in Immunoprecipitation (IP) — FASN antibody immunoprecipitates FASN protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 µg whole cell lysate/extract of FASN protein expressing HeLa cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of FASN by 2.5 µg of FASN antibody (Product # PA5-22061) 5% SDS-PAGE The immunoprecipitated FASN protein was detected by FASN antibody (Product # PA5-22061) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.

FASN Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in HepG2 Cas9 cell line transduced with FASN Lentiviral sgRNA compared to control cell line using Anti-FASN Polyclonal Antibody (Product # PA5-22061). {KO}

Product Details

PA5-22061

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 7 and 287 of Fatty Acid Synthase (Uniprot ID#P49327) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11156419

Product Specific Information

Recommended positive controls: Molt-4, mouse brain, PC-12, Rat2, 293T.

Predicted reactivity: Mouse (89%), Rat (89%), Pig (83%), Bovine (85%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.

Scientific reports

An M,Park YH,Lim YH

PA5-22061 was used in Western Blotting to investigate the antiobesity and antidiabetic effects of the dairy bacterium P. freudenreichii MJ2 isolated from raw milk using 3T3-L1 cells and high-fat diet (HFD)-induced obese mice.

Thu Jan 28 00:00:00 EST 2021

Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.

Scientific reports

An M,Park YH,Lim YH

Thu Jan 28 00:00:00 EST 2021

Bioinformatics

Protein Aliases: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; Acyl-[acyl-carrier-protein] hydrolase; Enoyl-[acyl-carrier-protein] reductase; Fatty acid synthase; MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; Type I FAS; Type I Fatty Acid Synthase

Gene Aliases: A630082H08Rik; FAS; FASN; OA-519; SDR27X1

UniProt ID: (Human) P49327, (Mouse) P19096, (Rat) P12785

Entrez Gene ID: (Human) 2194, (Mouse) 14104, (Rat) 50671

Function(s)

osteoblast differentiation acetyl-CoA metabolic process fatty acid metabolic process fatty acid biosynthetic process pantothenate metabolic process mammary gland development positive regulation of cellular metabolic process long-chain fatty-acyl-CoA biosynthetic process oxidation-reduction process cellular response to interleukin-4 cell-cell adhesion lipid metabolic process metabolic process biosynthetic process lipid biosynthetic process

Process(es)

FASN Polyclonal Antibody

Product Details

PA5-22061

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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