Proteintech

FECH Polyclonal Antibody

View all (9) FECH antibodies

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FECH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 14466-1-AP ( FECH Antibody) at dilution of 1:200 (under 40x lens).

FECH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 14466-1-AP ( FECH Antibody) at dilution of 1:200 (under 10x lens).

FECH Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FECH Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FECH Antibody in Western Blot (WB) — Human liver tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

FECH Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 14466-1-AP ( FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FECH Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FECH Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Product Details

14466-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FECH Fusion Protein Ag5814 (60-423 aa encoded by BC039841) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.18 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: VQPQKRKPK TGILMLNMGG PETLGDVHDF LLRLFLDRDL MTLPIQNKLA PFIAKRRTPK IQEQYRRIGG GSPIKIWTSK QGEGMVKLLD ELSPNTAPHK YYIGFRYVHP LTEEAIEEME RDGLERAIAF TQYPQYSCST TGSSLNAIYR YYNQVGRKPT MKWSTIDRWP THHLLIQCFA DHILKELDHF PLEKRSEVVI LFSAHSLPMS VVNRGDPYPQ EVSATVQKVM ERLEYCNPYR LVWQSKVGPM PWLGPQTDES IKGLCERGRK NILLVPIAFT SDHIETLYEL DIEYSQVLAK ECGVENIRRA ESLNGNPLFS KALADLVHSH IQSNELCSKQ LTLSCPLCVN PVCRETKSFF TSQQL (60-423 aa encoded by B C039841)

Target Information

The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.

Cell metabolism

Maio N,Kim KS,Singh A,Rouault TA

Tue Apr 04 00:00:00 EDT 2017

Involvement of protoporphyrin IX accumulation in the pathogenesis of isoniazid/rifampicin-induced liver injury: the prevention of curcumin.

Xenobiotica; the fate of foreign compounds in biological systems

He L,Guo Y,Deng Y,Li C,Zuo C,Peng W

Wed Feb 01 00:00:00 EST 2017

Heme levels are increased in human failing hearts.

Journal of the American College of Cardiology

Khechaduri A,Bayeva M,Chang HC,Ardehali H

Tue May 07 00:00:00 EDT 2013

MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes.

Journal of the American Heart Association

Qiao A,Khechaduri A,Kannan Mutharasan R,Wu R,Nagpal V,Ardehali H

Mon Apr 22 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Ferrochelatase, mitochondrial; Heme synthase; heme synthetase; Protoheme ferro-lyase; protoporphyria

Gene Aliases: AI894116; EPP; FCE; fch; Fcl; FECH

UniProt ID: (Human) P22830, (Mouse) P22315

Entrez Gene ID: (Human) 2235, (Rat) 361338, (Mouse) 14151

Molecular Function: lyase

generation of precursor metabolites and energy heme biosynthetic process response to light stimulus response to lead ion response to insecticide response to drug response to ethanol protoporphyrinogen IX metabolic process response to arsenic-containing substance response to methylmercury response to platinum ion cellular response to dexamethasone stimulus porphyrin-containing compound biosynthetic process cholesterol metabolic process detection of UV response to metal ion regulation of gene expression regulation of eIF2 alpha phosphorylation by heme erythrocyte differentiation very-low-density lipoprotein particle assembly heme metabolic process regulation of hemoglobin biosynthetic process iron ion homeostasis

Process(es)

ferrochelatase activity protein binding ferrous iron binding 2 iron, 2 sulfur cluster binding iron ion binding heme binding iron-responsive element binding tetrapyrrole binding lyase activity metal ion binding iron-sulfur cluster binding