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Invitrogen
FECH Polyclonal Antibody
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FIGURE: 1 / 5
FECH Antibody (PA5-97044) in IHC (P)
Immunohistochemistry analysis of FECH in paraffin-embedded mouse stomach. Samples were incubated with FECH Polyclonal antibody (Product # PA5-97044) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-97044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FECH (NP_0001312)
Conjugate
Form
Liquid
Concentration
0.76 mg/mL
Amount
76 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MRSLGANMAA ALRAAGVLLR DPLASSSWRV CQPWRWKSGA AAAAVTTETA QHAQGAKPQV QPQKRKPKTG ILMLNMGGPE TLGDVHDFLL RLFLDRDLMT LPIQNKLAPF IAKRRTPKIQ EQYRRIGGGS PIKIWTSKQG EGMVKLLDEL SPNTAPHKYY IGFRYVHPLT EEAIEEMERD GLERAIAFTQ YPQYSCSTTG; Positive Samples: HepG2, HT-29, Mouse brain; Cellular Location: Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Target Information
The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ferrochelatase, mitochondrial; Heme synthase; heme synthetase; Protoheme ferro-lyase; protoporphyria; unnamed protein product
Gene Aliases: AI894116; EPP; EPP1; FCE; fch; Fcl; FECH
UniProt ID: (Human) P22830, (Mouse) P22315
Entrez Gene ID: (Human) 2235, (Rat) 361338, (Mouse) 14151
ferrochelatase activity
iron ion binding
protein binding
ferrous iron binding
lyase activity
heme binding
iron-responsive element binding
identical protein binding
protein homodimerization activity
metal ion binding
tetrapyrrole binding
iron-sulfur cluster binding
2 iron, 2 sulfur cluster binding
lyase
generation of precursor metabolites and energy
porphyrin-containing compound biosynthetic process
heme biosynthetic process
heme a biosynthetic process
heme B biosynthetic process
cholesterol metabolic process
response to xenobiotic stimulus
response to light stimulus
detection of UV
response to metal ion
response to lead ion
response to insecticide
erythrocyte differentiation
very-low-density lipoprotein particle assembly
response to ethanol
protoporphyrinogen IX metabolic process
response to arsenic-containing substance
regulation of hemoglobin biosynthetic process
heme O biosynthetic process
response to methylmercury
multicellular organismal iron ion homeostasis
response to platinum ion
cellular response to dexamethasone stimulus
regulation of gene expression
regulation of eIF2 alpha phosphorylation by heme
heme metabolic process
response to drug
iron ion homeostasis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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