Invitrogen

FEN1 Polyclonal Antibody

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FEN1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of FEN1 was performed in HeLa cells using FEN1 Polyclonal Antibody (Product # PA5-88244).

FEN1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of FEN1 in paraffin-embedded human lung cancer using FEN1 Polyclonal Antibody (Product # PA5-88244) at a dilution of 1:100.

FEN1 Antibody in Western Blot (WB) — Western Blot analysis of FEN1 in extracts of various cell lines using FEN1 Polyclonal Antibody (Product # PA5-88244) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

FEN1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of FEN1 was performed in 200 µg extracts of HeLa cells using FEN1 Polyclonal Antibody (Product # PA5-88244). Western blot was performed from the immunoprecipitate using FEN1 Polyclonal Antibody.

Product Details

PA5-88244

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.96 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2804770

Product Specific Information

Immunogen sequence: GDVLQNEEGE TTSHLMGMFY RTIRMMENGI KPVYVFDGKP PQLKSGELAK RSERRAEAEK QLQQAQAAGA EQEVEKFTKR LVKVTKQHND ECKHLLSLMG IPYLDAPSEA EASCAALVKA GKVYAAATED MDCLTFGSPV LMRHLTASEA KKLPIQEFHL SRILQELGLN QEQFVDLCIL LGSDYCESIR GIGPKRAVDL IQKHKSIEEI VRRLDPNKYP VPENWLHKEA HQLFLEPEVL DPESVELKWS EPNEEELIKF MCGEKQFSEE RIRSGVKRLS KSRQGSTQGR LDDFFKVTGS LSSAKRKEPE PKGSTKKKAK TGAAGKFKRG K; Cellular Location: Mitochondrion, Nucleus, nucleolus, nucleoplasm

Target Information

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNase IV; FEN-1; FEN1; Flap endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; Maturation factor 1; maturation factor-1; MF1

Gene Aliases: AW538437; FEN-1; FEN1; MF1; RAD2

UniProt ID: (Human) P39748

Entrez Gene ID: (Human) 2237, (Mouse) 14156

Molecular Function: exodeoxyribonuclease