Proteintech

FER Polyclonal Antibody

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FER Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells using 25287-1-AP (FER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

FER Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25287-1-AP ( FER Antibody) at dilution of 1:50 (under 40x lens).

FER Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP ( FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FER Antibody in Flow Cytometry (Flow) — 1X10^6 Jurkat cells were stained with 0.5ug FER antibody (25287-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

FER Antibody in Immunoprecipitation (IP) — IP result of anti-FER (IP:25287-1-AP, 4ug; Detection:25287-1-AP 1:1000) with MCF-7 cells lysate 2400ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FER Fusion Protein Ag18173

Conjugate

Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells. FER is found in the cytoplasm and in the nucleus, where it was associated with the chromatin fraction. Its ubiquitous expression and its subcellular location indicate that it may be involved in key regulatory processes. The group IV cytoplasmic protein-tyrosine kinase Fer has been linked to cellular signaling responses to many different stimuli, including growth factors and cytokines. Data suggest a biological role for Fer in regulation of leukocyte recruitment during the innate immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 2.7.10.2; Feline encephalitis virus-related kinase FER; fer (fms/fps related) protein kinase, testis specific 2; fer (fps/fes related) tyrosine kinase; Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; p94-Fer; phosphoprotein NCP94; protein phosphatase 1, regulatory subunit 74; Proto-oncogene c-Fer; proto-oncogene tyrosine-protein kinase Fer; Tyrosine kinase 3; Tyrosine-protein kinase Fer

Gene Aliases: AV082135; C330004K01Rik; FER; Fert; Fert2; p94-Fer; PPP1R74; TYK3

UniProt ID: (Human) P16591, (Mouse) P70451

Entrez Gene ID: (Human) 2241, (Mouse) 14158

Function(s)