Immunofluorescent analysis of FERD3L in PFA-perfused cryosections of mouse embryo tissue using a FERD3L polyclonal antibody (Product# PA5-37986) at a dilution of 1ug/ml. Tissues were incubated overnight at 4°C and detection was performed using an Alexa Fluor 488-conjugated secondary antibody (green) with nuclear staining using DAPI (blue).
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-QFDERYQEVEGDE, from the internal region of Ferd3l (aa56-68)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.