Invitrogen

FGD1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (5) FGD1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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FGD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human cardiac tissue using an anti-FGD1 polyclonal antibody (Product # PA5-40416).

FGD1 Antibody in Western Blot (WB) — Western blot analysis of human Raji cell lysate using an anti-FGD1 polyclonal antibody (Product # PA5-40416).

FGD1 Antibody — Figure 4 FGD1 interacts with PTEN to inhibit PTEN phosphates activity in osteosarcoma cells. A, the WCL of 293T cells were subjected to silver staining and mass spectrometry after transfected indicated plasmids. B-C, 293T cells transfected with indicated plasmids was harvested for co-immunoprecipitation. D-E, Western blotting analysis of WCL of U-2OS, MG63 and MNNG/HOS cells. F, a schematic diagram depicting the domain of PTEN. G, Flag-FGD1, Myc-PTEN-C and Myc-PTEN-N were translated in vitro, and the co-immunoprecipitation was performed to evaluate the interaction between the PTEN recombination protein and FGD1. H, a schematic diagram depicting FGD1 interacted with the N-terminal region of PTEN. I, U-2OS, MG63 and MNNG/HOS cells were transfected with indicated constructs. After 72 h, the spend medium of each treatment group were collected for release phosphatase assay. ***, P < 0.001. J, Western blotting analysis of the WCL of osteosarcoma tumor cells (U-2OS, MG63 and MNNG/HOS) after transfected with indicated siRNAs. K, Western blotting analysis of the WCL of osteosarcoma tumor cells (U-2OS, MG63 and MNNG/HOS) after transfected with indicated plasmids. {KD}

Product Details

PA5-40416

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-1 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	4-8 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the C-terminal of human FGD1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2608209

Product Specific Information

Peptide sequence: WMAVLGRAGR GDTFCPGPTL SEDREMEEAP VAALGATAEP PESPQTRDKT

Sequence homology: Cow: 86%; Dog: 86%; Horse: 79%; Human: 100%; Mouse: 86%; Pig: 86%; Rabbit: 79%; Rat: 86%

Target Information

FGD1 contains Dbl (DH) and pleckstrin (PH) homology domains. It can bind specifically to the Rho family GTPase Cdc42Hs and stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. FGD1 has an essential role in embryonic development, and FGD1 gene mutations result in the human developmental disorder, Aarskog-Scott syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

FGD1 promotes tumor progression and regulates tumor immune response in osteosarcoma via inhibiting PTEN activity.

Theranostics

Wu W,Jing D,Meng Z,Hu B,Zhong B,Deng X,Jin X,Shao Z

Published figure using FGD1 polyclonal antibody (Product # PA5-40416) in Western Blot

Fri May 14 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Faciogenital dysplasia 1 protein; FYVE, RhoGEF and PH domain-containing protein 1; Rho/Rac GEF; Rho/Rac guanine nucleotide exchange factor FGD1; Zinc finger FYVE domain-containing protein 3

Gene Aliases: AAS; FGD1; FGDY; MRXS16; ZFYVE3

UniProt ID: (Human) P98174

Entrez Gene ID: (Human) 2245

Molecular Function: G-protein modulator protein-binding activity modulator guanyl-nucleotide exchange factor

Function(s)

cytoskeleton organization signal transduction multicellular organism development regulation of cell shape organ morphogenesis actin cytoskeleton organization regulation of Rho protein signal transduction positive regulation of apoptotic process regulation of GTPase activity positive regulation of GTPase activity filopodium assembly regulation of small GTPase mediated signal transduction

Process(es)

guanyl-nucleotide exchange factor activity Rho guanyl-nucleotide exchange factor activity small GTPase binding metal ion binding

FGD1 Polyclonal Antibody

Product Details

PA5-40416

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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