Invitrogen

FGF13 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (19) FGF13 antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Product Image Gallery

FGF13 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — FGF13 Polyclonal Antibody detects FGF13 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. FGF13 Polyclonal Antibody (Product # PA5-27302) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

FGF13 Antibody in Western Blot (WB) — Western blot analysis of FGF13 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a FGF13 Polyclonal Antibody (Product # PA5-27302) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

FGF13 Antibody — Sequence trace and alignment for CRISPR-mediated Fgf13 knockout. (A) Sequence trace for Fgf13 WT cells and Fgf 13 knockout clone 2H5. (B) Western blot analysis for Fgf13 WT cells, Fgf13 knockout cells, and Fgf13 add back cells. {KO}

Product Details

PA5-27302

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein fragment corresponding to a region within amino acids 1 and 176 of Human FGF13
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 1% BSA, 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2544778

Product Specific Information

Recommended positive controls: MCF-7.

Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (93%), Chicken (97%), Rhesus Monkey (97%), Chimpanzee (100%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is located in a region on chromosome X, which is associated with Borjeson-Forssman-Lehmann syndrome (BFLS), making it a possible candidate gene for familial cases of the BFLS, and for other syndromal and nonspecific forms of X-linked mental retardation mapping to this region. Alternative splicing of this gene at the 5' end results in several transcript variants encoding different isoforms with different N-termini.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

E2F1 Drives Breast Cancer Metastasis by Regulating the Target Gene FGF13 and Altering Cell Migration.

Scientific reports

Hollern DP,Swiatnicki MR,Rennhack JP,Misek SA,Matson BC,McAuliff A,Gallo KA,Caron KM,Andrechek ER

PA5-27302 was used in Western Blotting to uncover genomic features by which E2F1 regulates metastasis and identify pro-metastatic functions for the E2F1 target gene Fgf13.

Wed Jul 24 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: AFGF; ECGFA; ECGFB; FGF; FGF 1; FGF alpha; FGF-13; FGF13A; FGFA; FHF-2; Fibroblast growth factor; Fibroblast growth factor 13; Fibroblast growth factor homologous factor 2; GLIO703; HBGF 1; HBGF1

Gene Aliases: FGF-13; FGF13; FGF2; FHF-2; FHF2

UniProt ID: (Human) Q92913, (Rat) Q9ERW3, (Mouse) P70377

Entrez Gene ID: (Human) 2258, (Rat) 84488, (Mouse) 14168

Molecular Function: growth factor intercellular signal molecule