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          • Primary Antibodies ›
          • FGF22 Antibodies

          Invitrogen

          FGF22 Polyclonal Antibody

          View all (5) FGF22 antibodies

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          Cite FGF22 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          FGF22 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          FGF22 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          FGF22 Antibody (PA5-94992) in WB

          Western blot analysis of FGF22 in, Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          FGF22 Antibody in Western Blot (WB)
          FGF22 Antibody in Western Blot (WB)
          FGF22 Polyclonal Antibody

          Product Details

          PA5-94992

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to a sequence in the middle region of human FGF22 (93-109aa FYVAMNRRGRLYGSRLY).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 5mg BSA

          Contains

          0.05mg sodium azide, 0.05mg thimerosal

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2806798

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: human K562 whole cell, human Caco-2 whole cell, human U-87MG whole cell, human PC-3 whole cell, rat brain tissue, rat testicular tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The mouse homolog of this gene was found to be preferentially expressed in the inner root sheath of the hair follicle, which suggested a role in hair development. Alternative splicing results in multiple transcript variants.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: FGF; FGF-22; Fibroblast growth factor; Fibroblast growth factor 22

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          Gene Aliases: 2210414E06Rik; FGF-22; FGF22; UNQ2500/PRO5800

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          UniProt ID: (Human) Q9HCT0, (Mouse) Q9ESS2

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          Entrez Gene ID: (Human) 27006, (Mouse) 67112, (Rat) 170579

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          Function(s)
          fibroblast growth factor receptor binding growth factor activity receptor binding protein binding growth factor intercellular signal molecule
          Process(es)
          positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway neurogenesis cell differentiation regulation of cell migration positive regulation of MAPK cascade regulation of synapse maturation trans-synaptic signaling
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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